Background: Acquired immune deficiency syndrome (AIDS), can be defined as an infectious and fatal disease of the twentieth century, and caused by human immunodeficiency virus (HIV). The study aims to evaluate the effect of educational guidelines on knowledge of AIDS and its preventive practice among non-medical undergraduate students. Design: A true experimental pre-test-post-test research design with the control group was utilized in this study. Sampling Technique: Using purposive sampling, sixty students of College of Education, (thirty for each study and control group) between eighteen to twenty two years of age enrolled in third and fourth level female students had been participated in the study. Records had to be were gathered the use of an established self-administered questionnaire. Results: 83.3% of the study group and seventy percent of the control group were aged between eighteen to twenty years old, single and all of them were females. The mean general knowledge score about AIDS in the study group was increased from 7.47±2.44 in pretest to 14.5±2.1 in posttest with significant improvement was observed at p-value <0.001. The mean knowledge score about AIDS preventive methods in the study group was increased from 5.70 ± 2.72in pretests to 19.53 ± 0.86in posttest with significant improvement was observed at p-value <0.001. Conclusion & Recommendations: The study indicated that the educational guidelines have improved the knowledge and preventive practices on AIDs. Including in the educational curriculum about health education on AIDS/ HIV infection, which need lifestyle modifications in the current modern life. Key Words: AIDS, Educational guidelines, Knowledge and preventive practice.