Testicular cancer is the most common form of urogenital cancers among young men aged between 20–40 years. The incidence of testicular cancer is rapidly increasing. It is curable when detected and treated early. Aim: This study aimed to evaluate the effect of intervention guidelines on preventive behaviors among nursing males' students. Methods: A quasi-experimental study conducted at the technical institute of Beni-Suef university through the academic year (20182019). A convenient sample of (250) student male nurses have included in this study. Tools of data collection were a self-administrated questionnaire; Champion Health Belief Model Scale; testicular self-examination checklist, and student follow up card. Results: The current study revealed a mean age of students was 18.45 ± 1.65. The study showed that there was a significant increase in the mean score of satisfactory knowledge about testicular cancer and its preventive behaviors during follow up post-application of the intervention guideline at (p=0.001). Furthermore, an improvement in the testicular self-examination practices at post-intervention and follow (p=0.001). A statistically significant correlation revealed between the student nurses knowledge, practice, and preventive health believes and behaviors. Conclusion: The study concluded that intervention guideline designed based on the health belief model has a positive effect on promoting testicular cancer-preventive behaviors of student male nurses by improving their knowledge, practices, and health beliefs and behaviors. Recommendation dissemination of intervention guidelines among males at a different stage of life started from adolescents to reduce the risk of testicular cancer and its consequences on males reproductive health. Integrate the concept of TSE as a screening procedure for early detection of testicular cancer and other testicular disorders into the undergraduate curriculum of nursing faculties. Further research required to investigate barriers influencing the practice of testicular self-examination among Egyptian males. Keywords: Testicular cancer-preventive behaviors - Intervention guideline