Head trauma is a leading cause of disability and death in developing countries. The golden hour is a period of time lasting for one hour or less following a traumatic injury during which there is an urgent need for prompting care of the patients to improve outcomes and prevent death. The nurses’ performance plays a vital role in reducing the morbidity and mortality of patients with head trauma. This study aimed to evaluate the effect of education guidelines on nurses’ performance regarding patients with head trauma during golden hour at emergency department in Zagazig University hospitals. Research design: A quazi experimental design was utilized to achieve the aim of this study. Setting: The study was conducted in ED at emergency hospital, Zagazig University Hospitals. Sample: a convenience sample of all available nurses working in ED. Tools of data collection: Nurses’ Interviewing questionnaire, observational checklists, and nurses’ attitude Likert type rating scale were used. Results: 57.1% of the studied nurses aged more than 25yrs and 97% were male. There were statistically significant improvements in total nurses’ knowledge 65.7% compared to pre-test 14.3%, practice 77.1% post-test compared to pre-test 22.9%, and attitude 57.1% post-test compared to pre-test 8.6%. Conclusion: the education guidelines had a significant positive effect on improving nurses’ knowledge, practice, and attitude regarding patients with head trauma during a golden hour. It is recommended to continue in-service training programs for the purpose of refreshing and updating the nurse’s knowledge and practice regarding patients with head trauma during golden hour.