Abstract: Background: Intestinal ostomy care is a critical aspect of nursing practice, particularly in the care of patients with gastrointestinal disorders or those who have undergone surgical procedures such as colostomy or ileostomy. Aim of the study: Was to evaluate the effect of educational intervention on nurses’ performance and attitude regarding intestinal ostomy care at Suez Canal University Hospitals. Subjects and Methods: Research design: A quasi experimental design with pre-post test was conducted to achieve the aim of the study. Setting: The study was conducted at surgical departments at Suez Canal university hospitals, Egypt. Subjects: A convenient sample of all nurses (60 nurses) who performing actual patient care were included in the study from surgical departments at Suez Canal university hospitals. Tools of data collection: Three tools were used for data collection; Tool I: Nurses Interviewing Assessment Questionnaire. Tool II: Observational check list to assess nurses' practice regarding intestinal ostomy care. Tool III: Nurses Attitude Assessment Questioner. Results: There was a highly statistically significant difference between total nurses' level of knowledge about intestinal ostomy care pre and post educational intervention. As, there was (96.7%) of studied nurses had an un satisfactory level of knowledge pre intervention, while, (70.0%) of studied nurses had a satisfactory level of knowledge post intervention. In addition, there was a highly statistically significant difference between total nurses' level of practice about intestinal ostomy care pre and post educational intervention with P value of (0.0001). As there was (85%) of studied nurses had an incompetent level of practice pre intervention. While (68.3%) of studied nurses had a competent level of practice post intervention, Moreover, there was a highly statistically significant difference between nurses' attitude about intestinal ostomy care pre and post educational intervention with P value of (0.0001). As there was (96.7%) of studied nurses had a negative attitude pre intervention, While, (51.7%) of studied nurses had a positive attitude post intervention. Conclusion: There was a highly statistically significant positive effect of implementing the educational intervention on improving nurses' knowledge, practices and attitude toward providing intestinal ostomy care. The implemented educational intervention significantly enhanced nurses' performance toward intestinal ostomy care. Recommendations: Nurses need educational support for enhancing their knowledge, practices and attitude related to Intestinal ostomy care. Further research is needed to explore the long-term effects of educational interventions and their impact on patient outcomes.