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Prevalence and Distribution of Enterotoxin Genes among Bacillus cereus Isolated from Meat and Meat Products in Egypt
Faculty
Veterinary Medicine
Year:
2020
Type of Publication:
ZU Hosted
Pages:
Authors:
Ahmed Alsayed Tharwat
Staff Zu Site
Abstract In Staff Site
Journal:
Advances in Animal and Veterinary Sciences Advances in Animal and Veterinary Sciences
Volume:
Keywords :
Prevalence , Distribution , Enterotoxin Genes among Bacillus cereus
Abstract:
This study determined the incidence and distribution of two enterotoxin genes in Bacillus cereus isolates originating from meat and meat products. A total of 100 samples of meat, including minced meat, luncheon, sausage and pastrami (25 each), were randomly collected from different supermarkets at Gharbia Governorate, Egypt. The samples were cultured individually on mannitol-egg yolk-polymyxin (MYP) agar plates and sheep blood agar plates. Biochemical identification of presumptive colonies was performed. The cytotoxin K (cytK) and hemolysin BL (hblC) genes were detected in B. cereus strains (n=12) from minced meat and meat products by multiplex PCR. B. cereus was identified in 45% of the collected samples. A higher prevalence of B. cereus was detected in minced meat samples (76%) followed by luncheon (44%) and sausage (32%), while the lowest prevalence was observed in pastrami samples (28%). Pairwise comparisons showed significant differences in the distribution of B. cereus isolates between minced meat and pastrami samples (P=0.004), and minced meat and sausages(P=0.01). The co-occurrence of both cytK and hblC (66.7%) was dominant among enterotoxin genes, followed by cytK (25%) and hblC (8.3%). A higher incidence rate of B. cereus in minced meat indicates cross-contamination during processing and transportation, and therefore, represents a significant public health hazard for consumers
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Ahmed Alsayed Tharwat, "Prevalence and decontamination of Staphylococcus aureus in ready to eat chicken meat", Damanhour University, 2018
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Ahmed Alsayed Tharwat, "Hygienic status of meat served at hospitals and its improvement after HACCP implementation", Japanese Journal of Veterinary Research, 2019
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Department Related Publications
Mohammed Ezzat Ahmed Elsayed , "Characterization of different food‐isolated Enterococcus strains by MALDI‐TOF mass fingerprinting", USC-Spain, 2013
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Mohammed Ezzat Ahmed Elsayed , "Genetic discrimination of food-borne pathogenic and spoilage Bacillus spp. based on three housekeeping genes", Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences/College of Biotechnology, University of Santiago de Comp, 2015
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Eman Nabil Abdelfatah Mohammed, "Assessment of heavy metals content of some types of edible eggs in Sharkia, Egypt", First International Conference of Impact of Environmental Hazards on Food Safety, 2014
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Asmaa Badr Mostafa Badr, "Assessment of heavy metals content of some types of edible eggs in Sharkia, Egypt", First International Conference of Impact of Environmental Hazards on Food Safety, 2014
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Asmaa Badr Mostafa Badr, "Detection of enterotoxigenic Staphylococcus aureus in raw milk and cream using multiplex PCR", Faculty of Veterinary Medicine, Zagazig University, 2013
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