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High efficacy of a characterized lytic bacteriophage in combination with thyme essential oil against multidrug resistant Staphylococcus aureus in chicken products
Faculty
Veterinary Medicine
Year:
2020
Type of Publication:
ZU Hosted
Pages:
Authors:
Ahmed Alsayed Tharwat
Staff Zu Site
Abstract In Staff Site
Journal:
Iranian Journal of Veterinary Research, Shiraz University Shiraz University
Volume:
Keywords :
High efficacy , , characterized lytic bacteriophage in combination
Abstract:
Background: The emergence and spread of methicillin-resistant Staphylococcus aureus (MRSA) in the food industry have led to using alternative natural bioagents for controlling S. aureus in food. Aims: The current work aimed to isolate and characterize a lytic phage specific to S. aureus and evaluate its efficacy with thyme essential oil for controlling S. aureus growth in chicken fillets. Methods: Twenty S. aureus strains previously isolated from ready-to-eat chicken products were tested for antimicrobial susceptibility and used for phage isolation. Results: All S. aureus strains were multidrug-resistant (MDR). The isolated phage (vB_SauM_CP9) belonged to the family Myoviridae and maintained its stability at pH (4-9) and temperature (30-70°C). The phage showed lytic activity on ten S. aureus strains and had a burst size (228 PFU/infected cell), latent period (45 min), and rise period (15 min). A combination of S. aureus phage multiplicity of infection (MOI) 10 + thyme oil 1% caused a higher significant reduction in S. aureus growth (87.22%) in artificially inoculated chicken fillets than individual treatment with bacteriophage or thyme essential oil. Conclusion: To our knowledge, this is the first report to evaluate the efficacy of bacteriophage and thyme oil for controlling the growth of MDR S. aureus in chicken products and recommending application of S. aureus phage and thyme oil combination in the food industry to achieve food safety goals and consumer protection as well as mitigate the antimicrobial resistance crisis.
Author Related Publications
Ahmed Alsayed Tharwat, "Prevalence and decontamination of Staphylococcus aureus in ready to eat chicken meat", Damanhour University, 2018
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Ahmed Alsayed Tharwat, "Prevalence of multidrug-resistant Aeromonas hydrophila in crustaceans", Advances in Animal and Veterinary Sciences, 2022
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Ahmed Alsayed Tharwat, "Prevalence and Distribution of Enterotoxin Genes among Bacillus cereus Isolated from Meat and Meat Products in Egypt", Advances in Animal and Veterinary Sciences, 2020
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Ahmed Alsayed Tharwat, "Hygienic status of meat served at hospitals and its improvement after HACCP implementation", Japanese Journal of Veterinary Research, 2019
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Ahmed Alsayed Tharwat, "Health risk assessment of organochlorine pesticide residues in edible tissue of seafood", FRONTIERS, 2023
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