The effect of abattoir environment on the level of carcasses contamination was studied, this task was achieved after collection of 130 samples from Zagazig abattoir represented by hundred swabs of abattoir walls, abattoir floors, knives, worker’s hand, cattle and camel carcass surfaces. In addition to thirty water samples collected from the input water, carcass washing water, wastewater (10 of each). The prevalence of Escherichia coli (E.coli) was 60, 100, 30, 30, 00, 60, 100, 30, 70, 40, 60, 20, and 30%. Meanwhile the prevalence of Salmonella was 40, 70, 10, 00,00, 30, 80, 10, 40, 00, 20, 00, 10% in examined walls, floors, knives, worker’s hand, input water, washing water, waste water, cattle thigh, cattle shoulder, buffalo thigh, buffalo shoulder, camel thigh and camel shoulder, respectively. Enterohemorrhagic E.coli O26:H11 15/130 (11.53%) and Salmonella typhimurium 9/130 (6.92%) were predominant species among examined samples. Hundred percentage of isolated E.coli was resistant to penicillin and sensitivity was (77.8%) and (92%) for ciprofloxacin and gentamicin. Salmonella species showed 100% resistance to streptomycin and sensitivity was (77.4%) and (93.5%) for ciprofloxacin and gentamicin. Both of E.coli and Salmonella isolates showed multi antibiotic resistant (MAR). The public health importance of isolates was discussed