In a world where concrete structures face constant degradation from environmental forces, a revolutionary solution has emerged: bio-self-healing concrete. This innovation involves embedding dormant bacteria within the concrete mix, poised to spring into action when cracks form. As moisture seeps into the cracks, these bacterial agents are activated, consuming nutrients and converting them into calcium carbonate, a natural substance that fills and repairs the fractures, restoring the material’s integrity. This fascinating process represents a cutting-edge approach to maintaining concrete infrastructure, turning once-vulnerable materials into self-sustaining systems capable of healing themselves. The ongoing research into bio-self-healing concrete is focused on selecting bacterial strains that can withstand the extreme conditions within concrete, including its highly alkaline environment. The bacteria must also form resilient spores, remaining viable until they are needed for repair. Additionally, the study explores various challenges associated with this technology, such as the cost of production, the bacteria’s long-term viability, and their potential environmental impact. Advancements in genetic engineering and smart technology are being explored to enhance these bacterial strains, making them more efficient and robust in their role as microscopic repair agents. This review delves into the potential of bio-self-healing concrete to revolutionize how we approach infrastructure maintenance, offering a glimpse into a future where concrete structures not only endure but actively repair themselves, extending their lifespan and reducing the need for costly repairs.