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Extended-spectrum β-lactamase-producing E. coli from retail meat and workers: genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles
Faculty
Veterinary Medicine
Year:
2023
Type of Publication:
ZU Hosted
Pages:
10
Authors:
Heba Ahmed Abdallah Abdelmgeed
Staff Zu Site
Abstract In Staff Site
Journal:
BMC microbiology Springernature
Volume:
Keywords :
Extended-spectrum β-lactamase-producing E. coli from retail meat
Abstract:
Background The spread of extended-spectrum β-lactamases (ESBL) producing E. coli from food animals and the environment to humans has become a significant public health concern. The objectives of this study were to determine the occurrence, pathotypes, virulotypes, genotypes, and antimicrobial resistance patterns of ESBLproducing E. coli in retail meat samples and workers in retail meat shops in Egypt and to evaluate the bactericidal efficacy of silver nanoparticles (AgNPs-H2O2) against multidrug resistant (MDR) ESBL-producing E. coli. Results A total of 250 retail meat samples and 100 human worker samples (hand swabs and stool) were examined for the presence of ESBL- producing E. coli. Duck meat and workers’ hand swabs were the highest proportion of ESBLproducing E. coli isolates (81.1%), followed by camel meat (61.5%). Pathotyping revealed that the isolates belonged to groups A and B1. Virulotyping showed that the most prevalent virulence gene was Shiga toxin 2 (stx2) associated gene (36.9%), while none of the isolates harbored stx1 gene. Genotyping of the identified isolates from human and meat sources by REP-PCR showed 100% similarity within the same cluster between human and meat isolates. All isolates were classified as MDR with an average multiple antibiotic resistance (MAR) index of 0.7. AgNPs-H2O2 at concentrations of 0.625, 1.25, 2.5 and 5 μg/mL showed complete bacterial growth inhibition. Conclusions Virulent MDR ESBL-producing E. coli were identified in retail meat products in Egypt, posing significant public health threats. Regular monitoring of ESBL-producing E. coli frequency and antimicrobial resistance profile in retail meat products is crucial to enhance their safety. AgNPs-H2O2 is a promising alternative for treating MDR ESBLproducing E. coli infections and reducing antimicrobial resistance risks.
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Heba Ahmed Abdallah Abdelmgeed, "Bayesian Estimation of Diagnostic Accuracy of Three Diagnostic Tests for Bovine Tuberculosis in Egyptian Dairy Cattle Using Latent Class Models", MDPI, 2021
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Heba Ahmed Abdallah Abdelmgeed, "Occurrence, genotyping, shiga toxin genes and associated risk factors of E. coli isolated from dairy farms, handlers and milk consumers", Elsevier Ltd., 2016
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Heba Ahmed Abdallah Abdelmgeed, "A comparative evaluation of PCR- based methods for species- specific determination of African animal trypanosomes in Ugandan cattle", BioMed Central Ltd., 2013
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Heba Ahmed Abdallah Abdelmgeed, "Seafood a potential source of some zoonotic bacteria in Zagazig, Egypt, with the molecular detection of Listeria monocytogenes virulence genes", Istituto G. Caporale 2013, 2013
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Heba Ahmed Abdallah Abdelmgeed, "Cleavage site stability of Egyptian highly pathogenic avian influenza viruses in backyard chickens during 2009-2011", Elsevier B.V, 2014
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Department Related Publications
Alsaid Abozaid Abdelhadi Eldaly , "التقيم الكيميائى والحسى للأسماك المملحة أثناء فترة حفظها عند درجة 4 درجة مئوية", لايوجد, 1900
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Alsaid Abozaid Abdelhadi Eldaly , "بقايا الأريثروميسين فى لحوم الدواجن مع التركيز على تأثير بعض المعاملات على ثباته.", لايوجد, 1900
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Alsaid Abozaid Abdelhadi Eldaly , "تأثير طهى ثم تبريد الدواجن على تواجد الكلوستريديم برفرينجنز", لايوجد, 1900
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