Coliforms Contamination in Raw Milk and Some Dairy Products with Special Reference to Comparative Identification of Enterobacter spp.

Faculty Veterinary Medicine Year: 2019
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Zagazig Veterinary Journal Zagazig University Volume:
Keywords : Coliforms Contamination , , Milk , Some Dairy Products    
Abstract:
The current study was undertaken to examine 200 sample of raw milk and some dairy products (Kariesh cheese, plain yoghurt, milk powder and infant formula) for contamination with Coliform group especially Enterobacter spp. Coliforms were detected in; 42/50 (84%) raw milk samples from farmers' houses, 25/30 (83.33%) kariesh cheese samples and 23/30 (76.67%) plain yoghurt samples, however, they could not be detected in any of raw milk samples from dairy shops, milk powder and infant formula samples. The mean values of coliforms in the examined samples were 2.80x106 ±0.73x105, 2.30×106 ±0.75×104 and 1.08×106 ± 1.50×104 cfu /ml or gm in raw milk from farmers' houses, kariesh cheese and plain yoghurt samples, respectively. The biochemically identified coliforms were E. aerogenes, E. agglomerans, E. cloacae, C. diversus, C. freundii, E. coli, K. oxytoca and K. pneumonia with respective percentages of; 1.19, 1.19, 2.38, 25, 15.48, 26.19, 13.09 and 15.48, in raw milk from farmers' houses, 6, 0, 2, 28, 10, 22, 0, and 32 in kariesh cheese, 0, 0, 2.12, 23.91, 0, 36.96, 26.09, 10.87 in plain yoghurt. Comparative identification of isolated Enterobacter spp. by standard biochemical methods and MALDITOF MS revealed that the total conformity of identification of Enterobacter strains between was 66.6%, where, it ranged from 50% to 100 for E. aerogenes and E. cloaca, respectively. However, the only identified E. agglomerans isolates from raw milk could not be confirmed by MALDI-TOF MS technique. It has been shown through these results that the presence of coliform and Enterobacter bacteria is an evidence of the lack of health requirements and thermal treatments of raw milk and some of its products (kariesh cheese and yoghurt).
   
     
 
       

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