Journal: |
Zagazig Veterinary Journal
Zagazig University
|
Volume: |
|
Abstract: |
The current study was undertaken to examine 200 sample of raw milk and some
dairy products (Kariesh cheese, plain yoghurt, milk powder and infant formula) for
contamination with Coliform group especially Enterobacter spp. Coliforms were detected
in; 42/50 (84%) raw milk samples from farmers' houses, 25/30 (83.33%) kariesh cheese
samples and 23/30 (76.67%) plain yoghurt samples, however, they could not be detected in
any of raw milk samples from dairy shops, milk powder and infant formula samples. The
mean values of coliforms in the examined samples were 2.80x106 ±0.73x105, 2.30×106
±0.75×104 and 1.08×106 ± 1.50×104 cfu /ml or gm in raw milk from farmers' houses,
kariesh cheese and plain yoghurt samples, respectively. The biochemically identified
coliforms were E. aerogenes, E. agglomerans, E. cloacae, C. diversus, C. freundii, E. coli,
K. oxytoca and K. pneumonia with respective percentages of; 1.19, 1.19, 2.38, 25, 15.48,
26.19, 13.09 and 15.48, in raw milk from farmers' houses, 6, 0, 2, 28, 10, 22, 0, and 32 in
kariesh cheese, 0, 0, 2.12, 23.91, 0, 36.96, 26.09, 10.87 in plain yoghurt. Comparative
identification of isolated Enterobacter spp. by standard biochemical methods and MALDITOF
MS revealed that the total conformity of identification of Enterobacter strains
between was 66.6%, where, it ranged from 50% to 100 for E. aerogenes and E. cloaca,
respectively. However, the only identified E. agglomerans isolates from raw milk could not
be confirmed by MALDI-TOF MS technique. It has been shown through these results that
the presence of coliform and Enterobacter bacteria is an evidence of the lack of health
requirements and thermal treatments of raw milk and some of its products (kariesh cheese
and yoghurt).
|
|
|