Coliforms Contamination in Raw Milk and Some Dairy Products with Special Reference to Comparative Identification of Enterobacter spp.

Faculty Veterinary Medicine Year: 2019
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Zagazig Veterinary Journal Zagazig University Volume:
Keywords : Coliforms Contamination , , Milk , Some Dairy Products    
Abstract:
The current study was undertaken to examine 200 sample of raw milk and some dairy products (Kariesh cheese, plain yoghurt, milk powder and infant formula) for contamination with Coliform group especially Enterobacter spp. Coliforms were detected in; 42/50 (84%) raw milk samples from farmers' houses, 25/30 (83.33%) kariesh cheese samples and 23/30 (76.67%) plain yoghurt samples, however, they could not be detected in any of raw milk samples from dairy shops, milk powder and infant formula samples. The mean values of coliforms in the examined samples were 2.80x106 ±0.73x105, 2.30×106 ±0.75×104 and 1.08×106 ± 1.50×104 cfu /ml or gm in raw milk from farmers' houses, kariesh cheese and plain yoghurt samples, respectively. The biochemically identified coliforms were E. aerogenes, E. agglomerans, E. cloacae, C. diversus, C. freundii, E. coli, K. oxytoca and K. pneumonia with respective percentages of; 1.19, 1.19, 2.38, 25, 15.48, 26.19, 13.09 and 15.48, in raw milk from farmers' houses, 6, 0, 2, 28, 10, 22, 0, and 32 in kariesh cheese, 0, 0, 2.12, 23.91, 0, 36.96, 26.09, 10.87 in plain yoghurt. Comparative identification of isolated Enterobacter spp. by standard biochemical methods and MALDITOF MS revealed that the total conformity of identification of Enterobacter strains between was 66.6%, where, it ranged from 50% to 100 for E. aerogenes and E. cloaca, respectively. However, the only identified E. agglomerans isolates from raw milk could not be confirmed by MALDI-TOF MS technique. It has been shown through these results that the presence of coliform and Enterobacter bacteria is an evidence of the lack of health requirements and thermal treatments of raw milk and some of its products (kariesh cheese and yoghurt).
   
     
 
       

Author Related Publications

  • Asmaa Salah Mouhamd, "Occurrence of Bacillus cereus in raw milk and some dairy products in Egypt", Japanese Journal of Veterinary Research 64(Supplement 2): S95-102, 2016., 2016 More
  • Asmaa Salah Mouhamd, "Diversity of lactic acid bacteria isolated from raw milk inElsharkia province, Egypt", Japanese Journal of Veterinary Research 64(Supplement 2): S23-30, 2016., 2016 More
  • Asmaa Salah Mouhamd, "Effect of milking techniques on milk composition and sanitary condition of milk in El Sharkia Governorate", Journal of the Egyptian Veterinary Medical Association., 2013 More
  • Asmaa Salah Mouhamd, "Prevalence, molecular characterization and antimicrobial resistance of L. monocytogenes isolated from raw milk and dairy products.", Zagazig university, 2014 More
  • Asmaa Salah Mouhamd, "SCREENING OF SOME DAIRY PRODUCTS IN EGYPT FOR AFLATOXIN-M1 CONTAMINATION AND SOME HEAVY METAL RESIDUES.", Springer, 2017 More

Department Related Publications

  • Alsaid Abozaid Abdelhadi Eldaly , "التقيم الكيميائى والحسى للأسماك المملحة أثناء فترة حفظها عند درجة 4 درجة مئوية", لايوجد, 1900 More
  • Alsaid Abozaid Abdelhadi Eldaly , "بقايا الأريثروميسين فى لحوم الدواجن مع التركيز على تأثير بعض المعاملات على ثباته.", لايوجد, 1900 More
  • Alsaid Abozaid Abdelhadi Eldaly , "تأثير طهى ثم تبريد الدواجن على تواجد الكلوستريديم برفرينجنز", لايوجد, 1900 More
  • Alsaid Abozaid Abdelhadi Eldaly , "تواجد بعض ميكروبات التسمم ألغذائى فى بعض الأطعمة المجهزة الاستهلاك", لايوجد, 1900 More
  • Alsaid Abozaid Abdelhadi Eldaly , "بقايا المبيدات الحشرية الهيدروكلونية فى أنسجة الدجاج النيىء والمطبوخ وعلاقتها بالصحة العامة.", لايوجد, 1900 More
Tweet