n this work, pesticide residues in 493 fruit and vegetable samples obtained from markets in Turkey were detected after QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction followed by liquid chromatography-tandem mass spectrometry with electron spray ionization (LC-ESI/MS/MS) and gas chromatography-tandem mass spectrometry (GC/MS/MS). Validation of the method was tested based on the European Union SANTE/12682/2019 guidelines. The samples were analyzed to determine the concentrations of 500 pesticide residues. The results indicated that 254 samples of 493 samples contaminated with pesticides, only 22% contained pesticide residues at or below maximum residue limits (MRLs), and 30% contained pesticide residues above MRLs. Chlorpyrifos was the most common pesticide (105 samples) from the detected pesticides; 49 samples were found above to MRLs with concentrations of 0.011–2.001 mg/kg. Among samples, peach (88%), dill (84%), mushroom (83%), arugula (73%), and spinach (72%) were the crops with the higher percentages of pesticide residues.