Our study was applied to differentiate between S. enteritidis (Salmonella enteritidis) and S. typhimurium (Salmonella typhimurium) because of their importance for public health, epidemiologists, and the poultry industry, and to assess their pathogenic potential and antimicrobial resistance. For this purpose a total of 135 different food samples was studied, where only 8 Salmonella isolates were detected. The presence of virulence and antimicrobial resistance genes associated with the isolated strains was investigated by PCR, and the results of the analysis of five virulence genes showed that 100% of the examined strains were positive for invA, stn, fimH, and spvC genes, while only 62.5% were positive for pefA gene. Regarding the antimicrobial resistance, the highest resistance frequencies of the isolates were to ampicillin followed by nalidixic acid. All ampicillin-resistant salmonella isolates harbored the blaTEM gene, while none of these isolates carried the blaSHV gene.