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Search Result For 'LC-MS' , Result Number : 2

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Mai Ahmed Mohamed Ahmed alsayed
- Faculty Research Area On Zu Site
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LC-MS
Walid Rezk Alghareeb Elsaied
- Faculty Research Area On Zu Site
- Faculty Research Area On Staff Site
ANTIBIOTIC RESIDUE LEVELS IN CAMEL, CATTLE AND SHEEP TISSUES USING LC-MS/MS METHOD W. R. El-Ghareeb1,2, Z. Sh. Mulla1, A. M. A. Meligy3,4, W. S. Darwish2 and A. M. Edris1 1Department of Veterinary Public Health and Animal Husbandry, College of Veterinary Medicine, King Faisal University, Saudi Arabia 2 Food Control Department, Faculty of Veterinary Medicine, Zagazig University 44519, Egypt 3Department of Clinical Studies, College of Veterinary Medicine, King Faisal University, Saudi Arabia 4Physiology Department, Plant Protection Research Institute (PPRI), Agricultural Research Centre (ARC), Giza, Egypt Abstract Antimicrobials are very essential in prevention and control of infectious diseases for both humans and animals. However, the unregulated use of the antimicrobials may lead to several adverse health effects, such as development of multidrug resistant microbial strains, allergic and anaphylactic reactions. Saudi Arabia is one of the major countries in Middle East and Arabian Peninsula with a drastic increase in the livestock production. However, there is a clear lack of information about the current situation of antimicrobial residues in meat and edible offal intended for human consumption in Saudi Arabia. Therefore, this study screened the residue levels of nine antimicrobials with the most common use in Saudi Arabia veterinary medical field. These antimicrobials were enrofloxacin, ciprofloxacin, tylosin, erythromycin, tetracycline, oxytetracycline, chlortetracycline, sulfamethazine and sulfaquinoxaline. The tested samples included muscles, livers and kidneys of camel, cattle and sheep slaughtered at Al-Ahsa, Saudi Arabia. Antimicrobial residues in the tested samples was quantitatively estimated using liquid chromatography tandem mass spectrometry (LC–MS/MS). Sulfamethazine was detected at higher levels compared with the maximum residual limits (MPL) established by the regulatory authorities. Therefore, the effect of different cooking methods on sulfamethazine residues was investigated. The achieved results indicated occurrence of antimicrobial residues in the examined samples at levels below the recommended MPL, except for sulfamethazine that was detected at higher levels. Six antimicrobial residues were detected in samples collected from sheep; however, only three antimicrobials were detected in camel and cattle. Efficient heat treatment of the meat contaminated with sulfamethazine significantly reduced the residue load of sulfamethazine. In conclusion, efficient cooking of meat and observing the withdrawal periods of different antimicrobials are advisable to reduce human exposure to antimicrobial residues via meat consumption. مكان النشر: The Journal of Animal and Plant Sciences, August (2019).