The experimental work was conducted at Postharvest Laboratory of department of Floriculture Research, Horticulture Research Institute, Agriculture Research Center, Giza Egypt during the three successive seasons of 2001, 2002 and 2003. the aims of this work were to investigate the effect of some pulsing solutions and cold storage on longevity of tuberose cut flower. The important results of this work could be summarize as following:1- Effect of pulsing solutions:1.1- Using neem oil as pulsing solution for 24 hrs. at 1m/l for tuberose spikes then placing it in preservative solution 200 mg/l 8-HQS + 150 ml/l citric acid resulted in increase for longevity period similar to using STS 1-4 mM for 10 minutes then kinetin at 5 ppm for one night as pulsing solution. Increasing neem oil concentration to 2 or 3 ml/l was less effective in this regard.1.2- Using neem oil as pulsing solution for 24 hrs. resulted in significant increase in floret diameter. The best concentration was 3 ml/l where 2 or 1 ml/l were intermediate and the least value resulted from using 0.5ml/l. The intermediate concentrations of neem oil as 1 or 2 ml/l were similar in their effect to STS+Ki pulsing solution.1.3- Using neem oil at 0.5-3 ml/l as pulsing solution for 24 h resulted in high and early opening percentage for the florets comparing to STS + Ki as pulsing solution.1.4- The preservative solution uptake recorded high values when STS + Ki was used as pulsing solution comparing to neem oil.1.5- Using neem oil at 0.5 ml/l was effective in enhancing the increase percentage of fresh weight of flower stalk. This concentration was similar for using STS + Ki as pulsing solution. Less increase in the percentage of fresh weight was clear as neem oil concentration increased to 2 or 3 ml/l.1.6- STS + Ki or neem oil at 1 ml/L resulted in the highest soluble and total carbohydrates in the petals and were similar in this regard, whereas increasing neem oil to 3 ml/l was less effective.2- Effect of storage period and pre-storage pulsing with spermine:2.1- Storage period up to two weeks decreased significantly the longevity of tuberose. The reduction of longevity was diminished as the storage period decreased to 5 days.2.2- Pre-storage pulsing with spermine up to 5 ppm was not significantly effective in enhancing longevity of the stored spikes.2.3- Storage period for two weeks significantly decreased floret diameter. The magnitude of floret diameter decrease was less as storage period decreased to 5 or 10 days.2.4- The interaction between storage period and pre-storage pulsing with spermine was not significantly effective on floret diameter.2.5- Storage spikes for two weeks significantly decreased floret opening percentage to nearly third. Decreased storage period to 5 or 10 days only diminished this harmful effect.2.6- Pre-storage pulsing with spermine at 1 ppm for the spikes stored for 5 days resulted in the highest opening percentage.2.7- The spikes stored for two weeks showed less increase in fresh weight comparing to one week storage while storing spikes for 5 or 10 days attained increase in fresh weight percentage up to 6 days in the preservative solution. Prestorage pulsing with spermine at 1-5 ppm attained the increase in fresh weight percentage up to 10 days in the preservative solution for the spikes stored for 5 days only.2.8- Storing tuberose spikes for two weeks significantly decreased soluble, non soluble and total carbohydrates in the petals. Also decreasing storage period to 5 or 10 days showed similar significant reduction in carbohydrates percentage.2.9- Pre-storage pulsing with spermine at 1 or 2.5 ppm enhanced soluble, non soluble and total carbohydrates in the petals.2.10- The interaction between storage period and prestorage pulsing with spermine indicate that prestorage pulsing with spermine at 1 or 2.5 ppm improved soluble, non soluble and total carbohydrates percentage in the petals.