Background Nursing interns frequently encounter role ambiguity due to a mismatch between their expectations of the professional nursing role and the actual responsibilities they face in clinical settings. While clinical rotations during the internship year are intended to enhance clinical confidence and competence, such ambiguity can undermine these goals. Objective To examine the relationship between internship clinical rotation and role ambiguity among nursing interns. Methods A descriptive correlational study was conducted at Tanta University Hospitals, including six intensive care units, operating rooms, and general surgery departments. The study included all nursing interns (n = 900) enrolled in the internship year. Two tools were used to collect data: Tool I: Internship Clinical Rotation Fulfillment Questionnaire. Tool II: Role Ambiguity Questionnaire. Results Less than half of the nursing interns reported that clinical rotations fulfilled their intended goals, while approximately one-quarter indicated only moderate fulfillment. Interns expressed significant ambiguity regarding their role definition, role performance, training, and psychosocial support. A significant positive correlation (r = 0.380, p < 0.001) was found, reflecting that lower role ambiguity (reverse-scored) correlated with higher goal achievement. Conclusion The findings suggest that internship clinical rotations did not fully achieve their goals, and nursing interns experienced high levels of role ambiguity across multiple domains. Recommendations It is recommended that structured orientation programs be implemented for nursing interns, outlining job roles, hospital policies, and regulations. Additionally, clinical nurse educators should be trained in effective supervision practices, and consistent performance feedback should be provided to interns throughout their rotations.