Two phages were isolated from agricultural wastewater and soil were designated as Ps1 and Ps2. Host rang was restricted to Pseudomonas spp. The two phages were biologically characterized as belonging to Caudovirales, family Siphoviridae. Head diameters were 89.5 nm, 119 nm and the tails length were measured 230 and 112 nm for Ps1 and Ps2, respectively. Latent period was 15 min for both phages and rise period was 20 min for both phages. Burst sizes were 256 and 285 phage forming unit PFU/cell for Ps1 and Ps2 phages, respectively. Ps1 and Ps2 were completely inactivated after incubation at 84°C for 10 min, but were tolerant to alkaline as well as acidic reactions. Longevity in vitro (LIV) of Ps1 was 24 and 17 days at 22 ± 2 °C for Ps1 and Ps2, respectively. The RAPD-PCR results of Ps1 and Ps2 were 29 and 32 bands, respectively. Pseudomonas aeruginosa infected Nile talipa fish (Oreochromis niloticus) showed hemorrhages on the body surface, tail and fin rot, ulcerative skin, superficial ulcers, and full mortality through 14 days.