This study assessed the influence of supplementing the rabbit semen extender with various concentrations of glutathione (GSH) and taurine at 24, 48, and 72 h postchilling at 5°C. Semen samples were collected from 20 New Zealand bucks, and ejaculates with standard color, motility (>85%), about 0.5mL volume, and ∼400 ×106/mL concentration were used and diluted with extenders supplemented with 0.5, 1, and 2mM of GSH and 1, 5, and 10mM of taurine and chilled at 5°C. No supplemented samples were used as a control. Sperm’s progressive motility, acrosome reaction, and extracellular oxidative stress biomarkers such as MDA contents and GPx, SOD, and CAT concentrations and intracellular transcriptomic levels of SOD and CAT genes were assessed.