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Saudi Journal of Biological Sciences
elesivier
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Abstract: |
Different strategies have been approved for controlling extended-spectrum beta lactamase (ESBL) producing uropathogenic bacteria. The antibacterial activity of Lactic acid bacteria (LAB) is an effective strategy due to its probiotic characteristics and beneficial effects on human health. The antibiotic
susceptibility test, disk diffusion method, and double disc synergy test indicated that five enteric uropathogenic isolates were ESBL producers during the present study. They recorded diameters of inhibition
zones as 18, 8, 19, and 8 mm against cefotaxime (CTX), ceftazidime (CAZ), aztreonam (ATM), and
ceftriaxone (CRO). Genotypically, blaTEM genes are the most common, with (100 %) occurrence in all the
five enteric tested uropathogens, followed by blaSHV and blaCTX genes (60 %). In addition, out of 10 LAB
isolates from dairy products, the CFS of isolate no. K3 had high antibacterial activity against the tested
ESBLs, especially no. U60, with a MIC of 600 ll. Additionally, the MIC and sub-MIC of K3 CFS inhibited
the production of antibiotic-resistant bla TEM genes of U60. Analyzing the 16S rRNA sequence confirmed
that the most potent ESBL-producing bacteria (U60) and LAB (K3) isolates were identified as Escherichia
coli U60.1 and Weissella confuse K3 with accession numbers MW173246 and MW173299.1, respectively,
in GenBank
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