Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Attenuation of Oxidative Stress and Regulation of AKT Signaling by Vanillic Acid during Bovine Pre-Implantation Embryo Development
Faculty
Veterinary Medicine
Year:
2023
Type of Publication:
ZU Hosted
Pages:
Authors:
Ayman Atef Ahmed Abozied Mesalam
Staff Zu Site
Abstract In Staff Site
Journal:
Nutrients MDPI
Volume:
Keywords :
Attenuation , Oxidative Stress , Regulation , , Signaling , Vanillic
Abstract:
Vanillic acid (VA) has shown antioxidant and anti-inflammatory activities in different cell types, but its biological effects in the context of early embryo development have not yet been clarified. In the current study, the impact of VA supplementation during in vitro maturation (IVM) and/or post-fertilization (in vitro culture; IVC) on redox homeostasis, mitochondrial function, AKT signaling, developmental competence, and the quality of bovine pre-implantation embryos was investigated. The results showed that dual exposure to VA during IVM and late embryo culture (IVC3) significantly improved the blastocyst development rate, reduced oxidative stress, and promoted fatty acid oxidation as well as mitochondrial activity. Additionally, the total numbers of cells and trophectoderm cells per blastocyst were higher in the VA-treated group compared to control (p < 0.05). The RT-qPCR results showed down-regulation of the mRNA of the apoptosis-specific markers and up-regulation of AKT2 and the redox homeostasis-related gene TXN in the treated group. Additionally, the immunofluorescence analysis showed high levels of pAKT-Ser473 and the fatty acid metabolism marker CPT1A in embryos developed following VA treatment. In conclusion, the study reports, for the first time, the embryotrophic effects of VA, and the potential linkage to AKT signaling pathway that could be used as an efficacious protocol in assisted reproductive technologies (ART) to improve human fertility.
Author Related Publications
Ayman Atef Ahmed Abozied Mesalam, "Improves the In Vitro Developmental Competence and Reprogramming Efficiency of Cloned Bovine Embryos by Additional Complimentary Cytoplasm", Mary Ann Liebert, Inc., 2019
More
Ayman Atef Ahmed Abozied Mesalam, "Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology", MDPI, 2021
More
Ayman Atef Ahmed Abozied Mesalam, "Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition", Society for Reproduction and Fertility, 2017
More
Ayman Atef Ahmed Abozied Mesalam, "In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes", Elsevier, 2018
More
Ayman Atef Ahmed Abozied Mesalam, "Polydatin improves the developmental competence of bovine embryos in vitro via induction of sirtuin 1 (Sirt1).", CSIRO PUBLISHING, 2017
More
Department Related Publications
Abobakr Maher Elsayed Ali Hazaa, "The effect of different prophylactic regimes during pregnany on the subsequent postpartum fertility of friesian cows A. Hazzaa; AEidaroos; A. Bdel Raheim and H. Mansour", لايوجد, 1900
More
Abobakr Maher Elsayed Ali Hazaa, "Field observation on the uterine torsion in cows and buffaloes. A. Hazzaa; M.T. Nasr; A. Abdel Rahiem, A. Eidaroos and H. Mansour", لايوجد, 1900
More
Abobakr Maher Elsayed Ali Hazaa, "Investigation on the role played by the bulls in transmitting non-specific genital infection to cows and buffaloes in Egypt. El-Ghannam, F. Abdel-Raheim, A. Eidaroos, A.; Hazzaa, A. and Mansour, H", لايوجد, 1900
More
Abobakr Maher Elsayed Ali Hazaa, "Plasma Beta-endorphin profile duringt the different trimesters in Friesian dairy cows T.M. Barakat, A. Badr, G. Shawki and Hazzaa, A ", لايوجد, 1900
More
Abobakr Maher Elsayed Ali Hazaa, "Correlation among age body weight and scrotal circumference and some semen traits of growing Holstein Fresian bulls. Hazzaa, A ", لايوجد, 1900
More
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف