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Attenuation of Oxidative Stress and Regulation of AKT Signaling by Vanillic Acid during Bovine Pre-Implantation Embryo Development
Faculty
Veterinary Medicine
Year:
2023
Type of Publication:
ZU Hosted
Pages:
Authors:
Ayman Atef Ahmed Abozied Mesalam
Staff Zu Site
Abstract In Staff Site
Journal:
Nutrients MDPI
Volume:
Keywords :
Attenuation , Oxidative Stress , Regulation , , Signaling , Vanillic
Abstract:
Vanillic acid (VA) has shown antioxidant and anti-inflammatory activities in different cell types, but its biological effects in the context of early embryo development have not yet been clarified. In the current study, the impact of VA supplementation during in vitro maturation (IVM) and/or post-fertilization (in vitro culture; IVC) on redox homeostasis, mitochondrial function, AKT signaling, developmental competence, and the quality of bovine pre-implantation embryos was investigated. The results showed that dual exposure to VA during IVM and late embryo culture (IVC3) significantly improved the blastocyst development rate, reduced oxidative stress, and promoted fatty acid oxidation as well as mitochondrial activity. Additionally, the total numbers of cells and trophectoderm cells per blastocyst were higher in the VA-treated group compared to control (p < 0.05). The RT-qPCR results showed down-regulation of the mRNA of the apoptosis-specific markers and up-regulation of AKT2 and the redox homeostasis-related gene TXN in the treated group. Additionally, the immunofluorescence analysis showed high levels of pAKT-Ser473 and the fatty acid metabolism marker CPT1A in embryos developed following VA treatment. In conclusion, the study reports, for the first time, the embryotrophic effects of VA, and the potential linkage to AKT signaling pathway that could be used as an efficacious protocol in assisted reproductive technologies (ART) to improve human fertility.
Author Related Publications
Ayman Atef Ahmed Abozied Mesalam, "Improves the In Vitro Developmental Competence and Reprogramming Efficiency of Cloned Bovine Embryos by Additional Complimentary Cytoplasm", Mary Ann Liebert, Inc., 2019
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Ayman Atef Ahmed Abozied Mesalam, "Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology", MDPI, 2021
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Ayman Atef Ahmed Abozied Mesalam, "Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition", Society for Reproduction and Fertility, 2017
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Ayman Atef Ahmed Abozied Mesalam, "In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes", Elsevier, 2018
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Ayman Atef Ahmed Abozied Mesalam, "Polydatin improves the developmental competence of bovine embryos in vitro via induction of sirtuin 1 (Sirt1).", CSIRO PUBLISHING, 2017
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Department Related Publications
Abobakr Maher Elsayed Ali Hazaa, "Effect of intratesticular injection of prostaglandin F2? on some Aspects of Balady Buck semen. A. Hazzaa and Fattouh, El-S.M", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Total protein and its fractions in the blood serum and follicular fluid in she-camel. Eidaroos, A. Hazzaa, A. Nasr, M. and Abdel-Raheim, A ", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Evaluation of some Medicaments used to Improve reproductive performances of lactating cows. Barakat T. M.; Awad, H.H.; Hazzaa, A. and Ali, M.I.M", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Infleunce of age, body weight on some reproductive organs of growing Holstein Friestian bulls. G. Shawki, Hazzaa, A. and A. Badi ", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Correlation between reproductive and productive capacity of Friesian Holstein dairy cows. A. El-Marmi, K. Benhaj, Hazzaa, A., A. Badi M. Nasr and A. El-Katib", لايوجد, 1900
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