Tobacco smoke induces poor semen quality and male infertility. Therefore, we assess the effect of cigarettes on hormones of seminal plasma. Semen samples were obtained from forty tobacco married smoker men and forty strict married non-smoker men. Semen samples were subjected to: i) Analysis by a computer assisted semen analyzer. ii) Analysis for testosterone, follicular stimulating hormone and luteinizing hormone. Infertile smokers and non-smokers had significantly higher levels of FSH and LH compared to fertile smokers and non-smokers respectively. Level of testosterone was significantly lower in infertile smokers and nonsmokers compared to fertile smokers and non-smokers. In conclusion, our study has shown that infertile smoking men have reduced semen quality and altered hormonal level than infertile nonsmokers.