Bullying in nursing education has an extensive history and continues to be pervasive in the nursing profession. The aim of this study was determining the effect of bullying prevention program on nursing students' knowledge and the skills learned about bullying prevention. Materials and Methods: A quasi-experimental study was conducted in faculty of nursing Zagazig University. The sample included 81 nursing students in the fourth academic year, three tools were utilized 1) Bullying knowledge questionnaire, 2) Nursing student satisfaction with bullying prevention program, 3) General skills checklist. Results: The mean score of nursing students' knowledge about bullying in the study group increased throughout program phases (9.38 ± 9.67, 20.99 ± 7.02, and 20.99 ± 7.02), with a highly statistically significant difference in their knowledge about bullying in both pre-post, and pre-follow up phases of the program. In addition, the mean score of nursing students about general skills to deal with bullying increased gradually from situation one to situation five with a highly statistically significant difference between five situations (p = <0.001). Conclusion: Bullying program improved students’ knowledge and general skills; problem solving skills, assertiveness skills, stress management skills, and conflict management skills of nursing students.