Bullying in higher education is a growing problem which nursing students often witness and/or facing incidents of bullying within their faculty and clinical setting. The aim of this study was to assess nursing students' self- esteem and satisfaction with bullying prevention program. Materials and Methods: A cross-sectional study was conducted in faculty of nursing Zagazig University. The sample included 81 nursing students in the fourth academic year, A Two tools were utilized 1) Self-esteem scale 2) Nursing student satisfaction scale. Results: The mean score of nursing students' self-esteem increased throughout program phases (45.4±10.4, 60.7±9.03, and 61.3±7.73), with a highly statistically significant difference in their self-esteem in both pre-post, and pre-follow up phases of the program (p=0.001, & p=0.001 respectively). Also, the mean score of nursing students' satisfaction increased throughout program phases (36.8±16.7, 84.2±15.7, and 89.4± 5.6), with a highly statistically significant difference in their satisfaction in both pre-post, and pre-follow up phases of the program (p=0.000, & p=0.000 respectively). Conclusion: Nursing students satisfaction and self-esteem is higher after program implementation and after three months of program implementation than before bullying prevention program with a highly statistically significant difference throughout program phases. There is a need for introducing bullying prevention training at the fundamental level of all academic year in nursing courses.