The technique of random amplified polymorphic DNA (RAPD) has been used to detect DNA polymorphisms among E. granulosus isolates of camel, sheep, donkey and pigs. Eight arbitrary oligonucleotides (10- mer) were used as primers to amplify total genomic DNAs and significant genetic heterogeneity was detected among these isolates. Five of the Eight primers under assay, P1 followed by P2, P4, OPH-12 and P3 proved useful as genetic markers of strain variation, while P5,OPH-03 and OPH-18 allowed distinction at the genus level. P1, P2, P4 were the most effective primers giving sharp distinct banding pattern and the least values of similarity coefficient. some variations within E. granulosus isolates from the same type of the host were observed in form of differences in band intensities in all the primers used. The study implies that the donkeys are important host in transmission of hydatidosis in animals and human.