Background: Neonatal resuscitation” defined as the practice of helping to initiate breathing for neonates who can’t breathe spontaneously at birth. It is a proven method for avoiding harm from intrapartum-related hypoxic events. Aim of the study: Evaluate nurse's practice regarding neonatal resuscitation at Zagazig university hospitals. Subjects and method: Research design: A descriptive design was utilized in the present study. Setting: The study was conducted at neonatal intensive care unit and delivery room at Zagazig University Hospitals. Subjects: A convenient sample of 90 nurses. Tools of data collection: one tool was used in this study; it was Structured interview questionnaire and it was divided into two main parts: The first part was characteristics of the studied nurses. The second was nurse's practice about neonatal resuscitation. Results: the study showed that total scores of nurses' practice were 47% and 31% of studied nurses had fair and poor while only 22% had good practice. Conclusion: It was conducted that more than three quarter of the studied nurses had poor and fairly score level of practice regarding neonatal resuscitation. qualification, age, years of experience and training courses had highly significant associated with nurse's total practice scores Recommendation: the nurse’s practice regarding neonatal resuscitation be improved by continuous educational program and regular training. Key words: Resuscitation, Neonatal, Nurses practice