 Total RNA test could be used as indicator of individual variation for training programe.  D allele of ACE gene is related to strength and speed performance and α actinin 3 is related to white (type II) muscle fibers.  The increase in stem cells may be attributed as an elite horse.  CPK enzyme is a good marker of muscle damage whereas ALT and AST enzymes are indicators of soundness of muscle and liver.  Cortisol hormone could be used as indicator of stress in horse.