The 7S globulins (seed storage glycoprotein) isolated from soybean and chickpea seeds, has the antifungal potential against the pathogenic fungus (Botrytis cinerea) causing gray mold in grapes and were estimated either in vitro or postharvest table grapes by comparing with synthetic fungicide, Switch 62.5 WG. Conventional microbiological procedures such as linear growth curves and scanning electron microscopy (SEM) estimated the in-vitro antifungal potential of the 7S globulins. Soybean-7S significantly inhibited the in-vitro growth of Botrytis cinerea by about 64.44, 66.64, and 76.67% when applied at the rate of 50, 100, and 200 µg/mL, respectively, followed by chickpea 7S with growth reduction of 52.22, 54.44, and 66.67%, respectively. The synthetic fungicide (Switch 62.5 WG) induced higher growth inhibition extents amounting to 83.33 and 86.66% when applied at the rate of 50 and 200 µg/mL, respectively. The 7S-exposed B. cinerea displayed swollen hyphae compared to the control under scanning electron microscope examination. The 7S derived from soybean and chickpea inhibited the development of gray mold in table grapes when applied at 200 and 400 µg/mL for 30 days after infection with B. cinerea. The disease severity was also maintained at minimal levels (40 and 25%, for soybean-7S and chickpea-7S, respectively). Increased level of treatment (400 µg/mL), greatly reduced the disease severity down to only 7.5% after 30 days of storage at cold conditions for both proteins. The 7S globulin from legume seeds can be recommended as an alternative to synthetic fungicides for controlling B. cinerea as a postharvest treatment. These legume proteins could also be