Gray mold caused by Botrytis cinerea is the most harmful postharvest disease responsible for the degradation of strawberries. The present study aimed to prepare 7S-globulin from pea seeds, and to be used as an antifungal agent against B. cinerea in vitro and on the postharvest strawberry to extend the fruits' shelf life. The 7S-globulin was isolated from pea seed and characterized by various methods such as SDS-PAGE, FTIR, and pH solubility curve. The molecular technique was also used to confirm the identity of the causative microorganism of the grey mold disease in strawberries. By utilizing rRNA gene sequencing, a fungal pathogen that causes gray mold was identified as B. cinerea. The 7S-globulin showed three protein bands corresponding to α/ (83 KDa), α (68 KDa), and β (60 KDa) subunits. The isoelectric point was observed at pH 5.8. The essential and non-essential amino acids content occurred around 24.92%, and 54.04%, respectively. The 7S-globulin inhibited the mycelial growth of B. cinerea in a concentration-dependent manner. Scanning Electron microscope (SEM) of B. cinerea subjected to 7S-globulin showed swelling of both fungal hyphae and conidia, which were significantly affected by pea 7S-globulin treatment, entirely destabilizing and deforming their shape at 0.4 g/L. The 7S-globulin exposure also maintained fruit quality and stopped strawberry natural deterioration. Results further authenticated that 7S-globulin (isolated from pea seeds) was discovered with effective antifungal action against B. cinerea mycelial development via a membrane-targeted mechanism. The 7S-globulin affects hyphal morphology, compromises plasma membrane integrity, and prevented post-harvest grey mold on strawberry fruits.