Introduction and significance of the study: Tobacco use is one of the biggest public health concerns as it kills up to half of its long term users and is accountable for more than 8 million deaths worldwide each year. Moreover, tobacco use is considered a leading cause or contributor to several chronic health illnesses, including cancer, cardiovascular problems, respiratory problems, and stroke. The involvement of health professionals in tobacco-use prevention is considered one of the strategies to reduce smoking prevalence and health risks and to improve quality of life. Nurses form the largest cohort of healthcare professionals and spend many hours directly with patients. They can contribute to the reduction of tobacco use by assisting in smoking cessation endeavors. Since nurses form their professional roles while studying at a university or a college, it is important to examine their smoking history, behaviors, knowledge, attitudes, and beliefs toward smoking. Aim: The aim of this study was to investigate smoking knowledge, attitudes, beliefs, and behaviors toward tobacco smoking among undergraduate nursing program female students. Subjects and Methods: Research design: A cross-sectional, descriptive correlational design was used to achieve the study aims. Setting: The study was recruited from nursing schools in a governmental university located in Riyadh, the capital of Saudi Arabia. Subjects: A convenience sample of 134 female undergraduate nursing students. Data Collection Tools: A self-administered questionnaire, using questions from the Global Health Professional Student Survey (GHPSS), was utilized. Results: The sample ages range from 19 to 25 years, with a mean of 21.5 (±1.2) years. Eighty-three percent were single. About half of the students were in their third (53.7%) and fourth year (46.3%) of their nursing program. All subjects reported that tobacco smoking is harmful to health, and 73.9% (n = 99) believed that smoking causes nicotine addiction. Statistically significant differences between never smokers and ever smokers were found on “the knowledge on likelihood that an individual will avoid or decrease serious health problems when quitting smoking”. Thus, 73.1% of ever smokers compared with 94.4% of never smokers thought that it is ‘very likely’ or ‘likely’ that the individual who quits smoking would have lower chances to avoid or decrease serious health problems. Ever smokers reported more often (23.1%) allowance of smoking inside their home compared with never smokers (2.8%) (p < 0.01). The allowance of smoking in the presence of children among ever smokers was higher than never smokers. A significant statistical difference was shown between never smokers and ever smokers in their beliefs that formal stop-smoking program is a helpful method for smoking cessation (49.1% vs. 23.1%) (p= 0.02). Female nursing students were less likely to become smokers if they thought that a smoker who quits smoking would ‘very likely’ or ‘likely’ avoid or decrease serious health problems (p < 0.01). Conclusion: Knowledge about the harmful consequences of smoking alone was not enough to motivate smokers to quit. However, nursing students had positive attitudes against smoking and supported tobacco-banning measures. These attitudes are important as nurses play key roles in educating patients about the hazards of tobacco smoking and in providing smoking cessation interventions. Recommendations: The study recommended that nursing students should receive training on smoking cessation techniques since limited or lack of targeted smoking cessation training had been viewed as a barrier to implementing effective smoking cessation strategies.