Background: One of the most common human pathogens is the spiral and microaerophilic bacteria H. pylori, which also causes stomach adenocarcinoma, peptic ulcers, chronic gastritis, and mucosa-associated lymphoid tissue lymphoma in children. Mobile education based on smartphones is applied in many different areas. Due to its great portability, learning space is increased and learners can work at their own pace. Simulation learning is also possible. Information could be replayed. Objective: This study sought to assess the effect of a mobile-based teaching program on mothers' knowledge and reported practice regarding care of children with helicobacter pylori infection. Methods: The current study utilized a quasi-experimental design. The current investigation has been carried out in the outpatient pediatric clinics of Benha University Hospital as well as Benha Teaching Hospital. From the abovementioned setting, a non-probability purposeful sample of 100 women and their children was selected. Three tools have been utilized: (1) a structured interview questionnaire sheet; (2) a self-reported practice questionnaire sheet; and (3) a medication adherence scale. Results: Prior to the implementation of a mobile-based educational program, the majority of the examined mothers (89%) had an unsatisfactory level of knowledge; however, after the program, the vast majority (98%) had a satisfactory level of knowledge. The majority of the studied mothers (96%) had an inadequate level of practice in pre-program implementation. In contrast, 93% of them had an adequate level of practice post-program. Conclusion: Improvements in mothers' knowledge and practices regarding the care of their children with Helicobacter pylori infections were more effectively with mobile-based education. Again, there had been a highly statistically significant positive link between mothers' overall knowledge level, overall practice level, and overall medication adherence level before and after the mobile-based program's implementation. For mothers of children with Helicobacter pylori infection, healthcare practitioners could employ mobile-based educational methods to promote their children's health.