Background: Accidental poisoning is a leading cause of unintentional injuries among children in low-income and middle-income countries. Aim: This study aimed to assess nurses’ knowledge and practice toward children with accidental poisoning in the Poison Control Centers. Subjects & Methods: A cross-sectional descriptive study was conducted from 1st of September2021, to February 2022 at Zagazig Poison Control Centers (ZPCC) affiliated to Zagazig University Hospitals and Zagazig General Hospital, Sharqia Governorate, Egypt. A convenient sample of (n=95) nurses were enrolled in this study. Nurses' knowledge questionnaire and nurses' practice observational checklists were used for data collection. Results: less than half (47.4%) of studied nurses had poor knowledge about accidental poisoning, while 32.6% had average knowledge and 20% had good knowledge. Moreover, 61% studied nurses had unsatisfactory practice about accidental poisoning, while 39% had satisfactory practice. Conclusion: this study concluded that there was a high positive correlation between total knowledge and total practice at p value <0.01**. Recommendation: There are obvious needs for conducted educational training programs for nurses to improve their knowledge and practices regarding accidental poisoning in children.