Abstract: |
Column chromatography afforded the isolation of
seven secondary metabolites (1-(2,4,6-trihydroxy phenyl)-ethanone-4-O-β-D-glucopyranoside, naringenin-7-O-β-D-glucopyranoside, kaempferol-3-O-α-L-rhamnoside, kaempferol-3-O-β-D-glucopyranoside, quercetin-3-O-β-D-glucopyranoside, quercetin-3-O-βD-galactopyranoside, rutin) from the ethyl acetate (ET) fractions of
Morus macroura Miq. stems (S), leaves (L), and fruits (F). Their
identification based on ultraviolet (UV), electron ionization (EI),
electrospray ionization-mass spectrometry (ESI-MS), and 1D and
2D NMR data. In addition, profiling of ET fractions using
ultraperformance liquid chromatography-electrospray ionizationtandem mass spectrometry (UPLC-ESI-MS/MS) resulted in the
identification of 82 compounds belonging to different classes,
mainly polyphenolic constituents. Chemical profiling as well as
molecular docking directed us to biological evaluation. Interestingly, the ET-L fraction exhibited a robust cytotoxic activity against
HepG-2, MCF-7, and HELA cell lines. Also, it displayed a neuromodulatory activity against cisplatin neurotoxicity in rats by
ameliorating the neurobehavioral dysfunction visualized in the open field and Y-maze test and modulating the neurochemical
parameters such as brain amino acid levels (glutamate, aspartate, serine, and histidine), oxidative stress markers (GSH, MDA, and 8-
hydroxy-2′-deoxyguanosine), and purinergic cell energy (adenosine triphosphate (ATP) and adenosine monophosphate (AMP)). In
conclusion, the isolated compounds (kaempferol-3-O-β-glucoside and quercetin-3-O-β-glucoside) from the ET-L fraction co
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