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L‐Cysteine improves bovine oocyte developmental competence in vitro via activation of oocyte‐derived growth factors BMP‐15 and GDF‐9
Faculty
Veterinary Medicine
Year:
2022
Type of Publication:
ZU Hosted
Pages:
734-742
Authors:
Ayman Atef Ahmed Abozied Mesalam
Staff Zu Site
Abstract In Staff Site
Journal:
Reproduction in Domestic Animals WILEY
Volume:
7
Keywords :
L‐Cysteine improves bovine oocyte developmental competence
Abstract:
This study was designed to investigate the effect of different concentrations of L-cysteine supplementation into the maturation medium on the oocyte nuclear maturation, cumulus cell expansion, ultrastructure of the oocytes and the expression of oocyte-derived growth factors BMP-15, GDF-9 and CB-1 genes. Cumulus oocyte complexes (COCs) were collected from cow's ovaries obtained from abattoir and incubated at 38.5°C in maturation media supplemented with 0, 0.6, 0.8 or 1 mM L-cysteine in 5% CO2 under humidified air for 24 hr. We found that a significantly higher percentage of oocytes progressed to metaphase II stage in the in vitro maturation (IVM) medium supplemented with L-cysteine, particularly 0.8 mM group, compared with untreated control oocytes. Additionally, L-cysteine treatment significantly increased the number of expanded COCs and the degree of expansion of individual COCs. Results of RT-qPCR showed significant increase in expression levels of BMP-15 and GDF-9 in L-cysteine-treated groups compared with control one. Electron microgram showed improvement of cytoplasmic maturation regarding ultrastructure of the oocytes and oocyte-cumulus cell gap junction communication in all L-cysteine-treated groups especially 0.8 mM L-cysteine-treated one. In conclusion, supplementation of IVM medium with a potential anti-oxidant, L-cysteine can effectively improve in vitro oocytes cytoplasmic and nuclear maturation via activation of oocyte maturation related BMP-15 and GDF-9 genes in bovine oocytes, benefiting the extended researches about the potential applications of L-cysteine in mammalian breeding technologies.
Author Related Publications
Ayman Atef Ahmed Abozied Mesalam, "Improves the In Vitro Developmental Competence and Reprogramming Efficiency of Cloned Bovine Embryos by Additional Complimentary Cytoplasm", Mary Ann Liebert, Inc., 2019
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Ayman Atef Ahmed Abozied Mesalam, "Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology", MDPI, 2021
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Ayman Atef Ahmed Abozied Mesalam, "Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition", Society for Reproduction and Fertility, 2017
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Ayman Atef Ahmed Abozied Mesalam, "In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes", Elsevier, 2018
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Ayman Atef Ahmed Abozied Mesalam, "Polydatin improves the developmental competence of bovine embryos in vitro via induction of sirtuin 1 (Sirt1).", CSIRO PUBLISHING, 2017
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Department Related Publications
Abobakr Maher Elsayed Ali Hazaa, "Effect of intratesticular injection of prostaglandin F2? on some Aspects of Balady Buck semen. A. Hazzaa and Fattouh, El-S.M", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Total protein and its fractions in the blood serum and follicular fluid in she-camel. Eidaroos, A. Hazzaa, A. Nasr, M. and Abdel-Raheim, A ", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Evaluation of some Medicaments used to Improve reproductive performances of lactating cows. Barakat T. M.; Awad, H.H.; Hazzaa, A. and Ali, M.I.M", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Infleunce of age, body weight on some reproductive organs of growing Holstein Friestian bulls. G. Shawki, Hazzaa, A. and A. Badi ", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Correlation between reproductive and productive capacity of Friesian Holstein dairy cows. A. El-Marmi, K. Benhaj, Hazzaa, A., A. Badi M. Nasr and A. El-Katib", لايوجد, 1900
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