The Preservative Action of Protein Hydrolysates from Legume Seed Waste on Fresh Meat Steak at 4 °C: Limiting Unwanted Microbial and Chemical Fluctuations

Faculty Agriculture Year: 2022
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Polymers MDPI Volume:
Keywords : , Preservative Action , Protein Hydrolysates from Legume    
Abstract:
Valorizing agricultural wastes to preserve food or to produce functional food is a general trend regarding the global food shortage. Therefore, natural preservatives were developed from the seed waste of the cluster bean and the common bean to extend the shelf life of fresh buffalo meat steak and boost its quality via immersion in high-solubility peptides, cluster bean protein hydrolysate (CBH), and kidney bean protein hydrolysate (RCH). The CBH and the RCH were successfully obtained after 60 min of pepsin hydrolysis with a hydrolysis degree of 27–30%. The SDS-PAGE electropherogram showed that at 60 min of pepsin hydrolysis, the CBH bands disappeared, and RCH (11–48 kD bands) nearly disappeared, assuring the high solubility of the obtained hydrolysates. The CBH and the RCH have considerable antioxidant activity compared to ascorbic acid, antimicrobial activity against tested microorganisms compared to antibiotics, and significant functional properties. The CBH and the RCH (500 μg/mL) successfully scavenged 93 or 89% of DPPH radicals. During the 30-day cold storage (4 °C), the quality of treated and untreated fresh meat steaks was monitored. Protein hydrolysates (500 g/g) inhibited lipid oxidation by 130–153% compared to the control and nisin and eliminated 31–55% of the bacterial load. The CBH and the RCH (500 μg/g) significantly enhanced meat redness (a* values). The protein maintained 80–90% of the steak’s flavor and color (p < 0.05). In addition, it increased the juiciness of the steak. CBH and RCH are ways to valorize wastes that can be safely incorporated into novel foods.
   
     
 
       

Author Related Publications

  • Mohammad Namir Aldeen Mohammad Attia Alnemr, "Characterization of fiber pectin prepared from tomato processing wastes and its utilization in low-fat beef burger", قسم علوم الاغذية, 2008 More
  • Mohammad Namir Aldeen Mohammad Attia Alnemr, "Optimizing the Addition of Functional Plant Extracts and Baking Conditions To Develop Acrylamide-Free Pita Bread", Journal of food protection, USA, 2018 More
  • Mohammad Namir Aldeen Mohammad Attia Alnemr, "Acceleration of mackerel fish sauce fermentation via bromelain addition", Nutrition & Food Science, 2018 More
  • Mohammad Namir Aldeen Mohammad Attia Alnemr, "Acceleration of mackerel fish sauce fermentation via bromelain addition", Nutrition & Food Science, 2018 More
  • Mohammad Namir Aldeen Mohammad Attia Alnemr, "Upgrading Common Wheat Pasta by Fiber-Rich Fraction of Potato Peel Byproduct at Different Particle Sizes: Effects on Physicochemical, Thermal, and Sensory Properties", MDPI, 2022 More

Department Related Publications

  • Mohammed Ibrahim Sehata , "Possible protective role of nitric oxide in myocardial ischaemia", لايوجد, 1900 More
  • Mohammed Ibrahim Sehata , "Relative arteriovenous potency of nitric oxide", لايوجد, 1900 More
  • Mohammed Ibrahim Sehata , "The relationship between leptin levels and thyroid state in experimental animals.", لايوجد, 1900 More
  • Mohammed Ibrahim Sehata , "Age and sex related changes on cytokines.", لايوجد, 1900 More
  • Mohammed Ibrahim Sehata , "Orexin-A and some metabolic hormones.", لايوجد, 1900 More
Tweet