Proper soil management by application of rhizobacteria combined with eco-friendly materials as plant oils can significantly regulate of soil ecological processes by soil biota takes place through complex ecological interactions, consequently, change the structure and function of soil fauna. Here we investigated effectiveness of rhizobacteria and oils in vitro besides soil management under greenhouse and field conditions against root knot nematode, Meloidogyne incognita. Commercial rhizobacteria and four seed oils plants (colocynth, Citrullus colocynthis (L.), jojoba, Simmondsia chinensis (Link) Schneid., moringa, Moringa oleifera Lam. and marjoram oil (Origanum majorana L.) were tested in vitro on eggs and J2 of M. incognita using egg immobility and hatchability assay, larvicidal assay, as well as, in vivo under greenhouse and field conditions. Compared to the infected plants, significant effects (P ≤ 0.05) were observed for the tested oils stock solutions and recommended application rate (RC) of rhizobacteria against eggs and J2 of M. incognita in vitro. Under greenhouse conditions, curative application by oils and rhizobacteria significantly promoted fresh root, fresh shoot weight and leaves number of pepper plants than the control. Rhizobacteria and oxamyl resulted a significantly induced higher plant root and shoot weight percent than other tested materials compared with infected plants. As well as pots treated with rhizobacteria ranked next of oxamyl in decreasing numbers of galls and egg masses whereas pots treated with O. majorana showed the lowest nematicidal effect. Field evaluation of eco-friendly materials against M. incognita in naturally infected tomato plants revealed that repeated application by rhizobacteria and oils mixtures exhibited better results in declining galls numbers, egg masses, and J2 density in soil. These findings suggest that a possible mechanism by rhizobacteria and oils are able to manipulate plant parasitic nematodes behaviour in field soils and potentially able to reduce nematodes damage.