Abstract: Background: A healthy work environment for nurses is complex and multidimensional, comprised of numerous components and relationships among the components. The practice environment was defined as the organizational characteristics of the work environment that help or hinder professional nursing practice. It constitute important factors that can affect the quality and safety of care, such as autonomy, control over the environment and collaboration between physicians and nurses can make a practice environment more favorable of the development of care and also contribute to better outcomes for the patient, the nurses and the institution. Aim of the study: to assess the effects of nursing work environment and burnout in the safety climate, teamwork climate and job satisfaction. Subjects and Methods: Research design: A descriptive correlational study design was used. Setting: This study was conducted at Zagazig University Hospitals. Subjects: Stratified random sample of 384 nurses working at Zagazig University Hospitals. Tools of data collection: Three tools were used for data collection namely: the Korean version of the nursing work index–Revised scale (NWI-R), Maslach Burnout Inventory scale (MBI), and Safety Attitudes Questionnaire Short Form 2006 (SAQ). Results: Findings revealed that the highest mean percent scores were for safety climate, followed by nurses' work environment, while the lowest mean percent score was for job satisfaction. In addition, there were statistically significant positive correlations between nursing work environment, safety climate, teamwork climate, and job satisfaction. However, there were negative relations between Burnout and safety climate, teamwork climate, and job satisfaction with significant relation only with safety climate, and job satisfaction. Conclusion: nursing work environment was a good predictor of safety climate, teamwork climate, and job satisfaction. Additionally, burnout was a good predictor of safety climate, and job satisfaction, While was not predictor to teamwork climate. Recommendations: Hospitals' administrators and nursing managers should Implement and maintain education and training programs aimed at increasing awareness of healthy work environment and safety issues for nurses. Additionally, engage in knowledge transfer activities that promote best practices regarding the health, safety and well-being of nurses.