Newcastle disease caused by infection with the Newcastle virus is one of dangerous and important diseases that affect poultry and other avian species. It is a disease that affects all ages with and affects broiler as well as laying flocks, causing heavy losses as a result of infection, deaths and lack of egg production. This work was performed to assess the molecular and epidemiological status of the NDVs in different domesticated birds in Egypt. One hundred sixty-eight samples were obtained from distinct avian species, including turkeys, chickens, ducks, quail, pigeons, and ostriches during 2018-2020. After sample propagation on 9-days-old SPF ECE, NDVs presence were confirmed using conventional serological methods (HI) and molecular detection (RT-PCR) using primers targeting the Matrix and Fusion genes. The NDVs were confirmed in 20 samples (12.27%) out of 168 tested samples. Pathotyping of four selected positive isolates representing different investigated avian species revealed velogenic NDVs with ICPI was 1.67 to 1.96. Further characterization via using partial F gene phylogenetic and sequencing analysis revealed that the six tested isolates were had 112GRRQKR↓F117 at the cleavage site of Fusion protein. All six isolates were clustered with genotype VII1.1 NDVs via phylogenetic analysis. The identified nucleotide sequences among the six NDV isolates ranged from 94.9% to 100%. This study revealed the continuous circulation of velogenic NDVs in different avian species although the intensive use of vaccines against ND so regular monitoring and surveillance must be applied to decrease its adverse economic effect on poultry sector in Egypt. The aim of this study is to know the epidemiological status of the Newcastle virus in different vaccinated and non-vaccinated avian species in Egypt.