Food pathogens are causing many diseases with significant effects on human health and the economy. The Centers for Disease Control and Prevention (CDC) reported that approximately 48 million Americans get sick, 128,000 are hospitalized, and 3000 die each year from food poisoning. As specified by the CDC, the known pathogens (bacteria, viruses, and parasites) account for most foodborne illnesses, hospitalization, and death in the United States. Opuntia spp. are, to a great extent, dispersed in Africa, Asia, America, and the Mediterranean bowl. This plant is utilized as a dietary and pharmaceutical operator in a different dietary and value-added push. Even though contrasts within the phytochemical composition exist between wild and tamed (Opuntia ficus-indica) Opuntia spp., all Opuntia vegetative (pear, roots, cladodes, pulp, and seeds) display useful properties as anticancer and antimicrobial agents (flavonoids, and ascorbate), and as antimicrobial (phenolic acids) agents. Other phytochemical components (soluble fibers and biopeptides) have been shared in the antimicrobial character of Opuntia spp. Bioactive peptides are composed of a few amino acids linked by covalent bonds called amide bonds or peptides. Depending on their grouping of amino corrosive, these peptides may influence the body’s major frameworks such as resistant, stomach related, cardiovascular, and anxious system. The bioactive peptides can be utilized as valuable nourishment additives. Their grouping measures contrast from 2 to 20 amino corrosive buildups, but a few peptides have a long chain of amino corrosive (lunasin 43 amino corrosive). Bioactive peptides have the plausibility to be utilized as characteristic nourishment added substance and pharmaceuticals constituents to avoid nourishment harming and nourishment added items due to their antioxidant and/or antimicrobial impacts. Moreover, natural molecules from Opuntia spp. ought to be planned in vitro, in vivo, in situ, and in a way to assess the risks to humans, animals, and food chains. This chapter is an upgrade on the bioactive molecule’s properties of Opuntia spp. and their potential intrigued as antimicrobial.