Background: Recently, the consumption of cola markedly increased. So, the health effects of cola drinks are an important public matter. However, there are contradictory reports on its effects on hepatic, renal function. Aim of the study: To examine the effect of chronic Pepsi-Cola consumption on liver, kidney functions, their association to serum vitamin D (Vit.D) level and oxidative stress biomarkers in rats. Material, Methods: 32 adult male albino rats were divided equally into two groups: Group I: control group, Group II: chronic cola consumption group which had free access to cola beverage for 3 months. At the end of the study serum alanine aminotransferase(A.L.T.), serum aspartate aminotransferase(A.S.T), total proteins, albumin, globulin, urea, creatinine, Vit.D, calcium, phosphorus, glucose, insulin, malondialdehyde(M.D.A.), total antioxidant capacity(T.A.C.), glutathione(G.S.H.), superoxide dismutase(S.O.D.) levels were determined. Livers, kidneys were processed for histopathology. Results: There was a significant increase in serum A.S.T, A.L.T., urea, glucose, insulin, M.D.A. in cola group compared to control group. There was a significant decrease in serum Vit.D, T.A.C., G.S.H., S.O.D., calcium, accompanied by a significant increase in serum phosphorus. Additionally, there was a significant negative correlation between Vit.D and(A.S.T, A.L.T., M.D.A.) in cola group. However, there was a significant positive correlation between Vit.D and(T.A.C., G.S.H., S.O.D.) in cola group. Also, liver, renal sections showed histopathological changes. Conclusion: cola consumption induced Vit.D deficiency which was associated with oxidant/antioxidant imbalance that could explain its hazardous impact on liver, kidney functions in rats.