Abstract Background: Nurses are expected not only to have the appropriate nursing skills and knowledge but also to have the ethical and legal standards resulting from the standard practices. Aim: The aim of this study was to examine the effect of professional nursing ethics teaching program on Knowledge, attitudes and practice issues among nurses. Methods: A quasi-experimental design was utilized. Setting: The study was conducted at Zagazig University Emergency Hospital. Sample: Seventy staff nurses working in different departments of Zagazig University Emergency Hospital were recruited for the study. Tools of data collection: Three tools were used: (1) Nursing Ethics Knowledge Questionnaire, (2) Nursing Ethics Attitude Questionnaire, and (3) Issues in Practice of Nursing Ethics Questionnaire. Results: There were statistically significant strong correlations between nurses' knowledge and attitudes of professional nursing ethics after the teaching program, As well as a strong correlation between nurses' knowledge and practice issues of professional nursing ethics after the teaching program. Conclusion: professional nursing ethics teaching program has a positive influence in improving staff nurses' knowledge, attitudes and practice issues regarding professional nursing ethics. Recommendation: Nurses must actively participate in professional activities to expand knowledge, develop skills, understand the impact of ethical issues, and implement strategies to accomplish desired changes in the work environment while preserving personal integrity and authenticity. Keywords: Attitudes, Knowledge, Teaching program, Professional Nursing, Practice issues