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Graphene Oxide-Silver Nanoparticle Nanocomposites Induce Oxidative Stress and Aberrant Methylation in Caprine Fetal Fibroblast Cells
Faculty
Veterinary Medicine
Year:
2021
Type of Publication:
ZU Hosted
Pages:
Authors:
Ayman Atef Ahmed Abozied Mesalam
Staff Zu Site
Abstract In Staff Site
Journal:
Cells MDPI
Volume:
Keywords :
Graphene Oxide-Silver Nanoparticle Nanocomposites Induce Oxidative
Abstract:
Graphene oxide-silver nanoparticle (GO-AgNPs) nanocomposites have drawn much attention for their potential in biomedical uses. However, the potential toxicity of GO-AgNPs in animals and humans remains unknown, particularly in the developing fetus. Here, we reported the GO-AgNP-mediated cytotoxicity and epigenetic alteration status in caprine fetal fibroblast cells (CFFCs). In brief, the proliferation and apoptosis rate of GO-AgNP-treated CFFCs (4 and 8 µg/mL of GO-AgNPs) were measured using the cell-counting kit (CCK-8) assay and the annexin V/propidium iodide (PI) assay, respectively. In addition, the oxidative stress induced by GO-AgNPs and detailed mechanisms were studied by evaluating the generation of reactive oxygen species (ROS), superoxide dismutase (SOD), lactate dehydrogenase (LDH), malondialdehyde (MDA), and caspase-3 and abnormal methylation. The expression of pro- and anti-apoptotic genes and DNA methyltransferases was measured using reverse transcription followed by RT-qPCR. Our data indicated that GO-AgNPs cause cytotoxicity in a dose-dependent manner. GO-AgNPs induced significant cytotoxicity by the loss of cell viability, production of ROS, increasing leakage of LDH and level of MDA, increasing expression of pro-apoptotic genes, and decreasing expression of anti-apoptotic genes. GO-AgNPs incited DNA hypomethylation and the decreased expression of DNMT3A. Taken together, this study showed that GO-AgNPs increase the generation of ROS and cause apoptosis and DNA hypomethylation in CFFCs. Therefore, the potential applications of GO-AgNPs in biomedicine should be re-evaluated.
Author Related Publications
Ayman Atef Ahmed Abozied Mesalam, "Improves the In Vitro Developmental Competence and Reprogramming Efficiency of Cloned Bovine Embryos by Additional Complimentary Cytoplasm", Mary Ann Liebert, Inc., 2019
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Ayman Atef Ahmed Abozied Mesalam, "Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology", MDPI, 2021
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Ayman Atef Ahmed Abozied Mesalam, "Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition", Society for Reproduction and Fertility, 2017
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Ayman Atef Ahmed Abozied Mesalam, "In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes", Elsevier, 2018
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Ayman Atef Ahmed Abozied Mesalam, "Polydatin improves the developmental competence of bovine embryos in vitro via induction of sirtuin 1 (Sirt1).", CSIRO PUBLISHING, 2017
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Department Related Publications
Abobakr Maher Elsayed Ali Hazaa, "The effect of different prophylactic regimes during pregnany on the subsequent postpartum fertility of friesian cows A. Hazzaa; AEidaroos; A. Bdel Raheim and H. Mansour", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Field observation on the uterine torsion in cows and buffaloes. A. Hazzaa; M.T. Nasr; A. Abdel Rahiem, A. Eidaroos and H. Mansour", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Investigation on the role played by the bulls in transmitting non-specific genital infection to cows and buffaloes in Egypt. El-Ghannam, F. Abdel-Raheim, A. Eidaroos, A.; Hazzaa, A. and Mansour, H", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Plasma Beta-endorphin profile duringt the different trimesters in Friesian dairy cows T.M. Barakat, A. Badr, G. Shawki and Hazzaa, A ", لايوجد, 1900
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Abobakr Maher Elsayed Ali Hazaa, "Correlation among age body weight and scrotal circumference and some semen traits of growing Holstein Fresian bulls. Hazzaa, A ", لايوجد, 1900
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