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Moringa oleifera leaves ethanolic extract influences DNA damage signaling pathways to protect liver tissue from cobalt -triggered apoptosis in rats
Faculty
Veterinary Medicine
Year:
2020
Type of Publication:
ZU Hosted
Pages:
Authors:
Samah Ramadan Elsayed Khallil
Staff Zu Site
Abstract In Staff Site
Journal:
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Elseiver
Volume:
Keywords :
Moringa oleifera leaves ethanolic extract influences
Abstract:
This study assessed the potential of Moringa oleifera leaves ethanol extract (MLEE) in attenuating the detrimental effects of cobalt dichloride (CoCl2) on rat liver. Forty rats were assigned to five equal groups: control group, MLEE-treated group, CoCl2-treated group, prophylaxis co-treated group, and therapeutic co-treated group. The levels of Co, hepatic injury markers, total antioxidant capacity (TAC), and oxidative stress biomarkers (reactive oxygen species [ROS] and protein carbonyl [PC]) were evaluated. Comet assay was used to evaluate the extent of DNA damage. Further, the expression profile of DNA-damage effector genes was assayed by real-time quantitative polymerase chain reaction (qRT-PCR) analysis. Immunohistochemical analysis of heat shock protein (HSP70) in hepatocytes was conducted. The results showed that the exposure of CoCl2 to rats resulted in declined TAC, elevated oxidative injury, and induced DNA damage markers. Upregulation of mRNA expression of tumor suppressor protein (P53), apoptosis inducing factor (AIF), and apoptotic peptidase activating factor 1 (Apaf-1) was observed. The immunostaining density of HSP-70 expression was found to be elevated. Thus, MLEE reduced the CoCl2-induced genotoxicity by preventing CoCl2-induced generation of ROS, and protected against ROS mediated-oxidative injury and DNA damage. Moreover, the expression of DNA damage effector genes was affected. Based on these results, we conclude that MLEE is more effective when administered as a prophylactic regimen with the exposure to CoCl2.
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Department Related Publications
Khairy Mohamed Albayoumi Mohamed, "دراسات على الجاموس المصرى"1- مقاييس الجسم وعلاقتها ببعض الصفات الإقتصادية Studies on Egyptian Buffaloes I. Body measurement as related to some economic traits", مجلة الإسكندرية للعلوم البيطرية – العدد (5) – رقم (2) – 1989, 1989
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Khairy Mohamed Albayoumi Mohamed, "تأثير السلاله والخصى للماعز على نسبة التصافى وصفات الذبيجه Effect of breed and castration of goats on dressing percentage and carcass traits", : مجلة أسيوط للعلوم الطبية البيطرية العدد (20) – رقم (39) – 1988, 1988
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Khairy Mohamed Albayoumi Mohamed, "تأثير بعض العوامل البيئية والوراثيه على مكونات اللبن لأبقار الفريزيان فى مصر Effect of some environmental and genetic factors on milk constitutes of Friesian cows in Egypt", مجلة الزقازيق للبحوث الزراعية – العدد (16) – رقم (2) – 1989, 1989
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Khairy Mohamed Albayoumi Mohamed, "دراسات على أبقار الفريزيان فى مصر "1- نمط النمو فى عجول الفريزيان من الولادة حتى عمر 12 شهر." "Studies on Friesian cattle in Egypt" "I. Growth pattern in Friesian calves from birth to tweleve month of age."", مجلة الزقازيق البيطرية – العدد (17) – رقم (2) – 1989., 1989
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Khairy Mohamed Albayoumi Mohamed, "تأثير عدد الخلفه وكثافة اعداد التربية على أوزان الجسم قبل وبعد الفطام فى الأرنب النيوزيلاندى الأبيض." Effect of litter size and stocking density on body weight of pre and post- weaning New Zealand White rabbits", المجلة الطبية البيطرية- الجيزة – كلية الطب البيطرى – جامعة القاهرة , 1993
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