Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Tramadol Promotes Oxidative Stress, Fibrosis, Apoptosis, Ultrastructural and Biochemical alterations in the Adrenal Cortex of Adult Male Rat with Possible Reversibility after withdrawal
Faculty
Medicine
Year:
2020
Type of Publication:
ZU Hosted
Pages:
Authors:
Mohamed Ali Ahmed Mohamed Alabiad
Staff Zu Site
Abstract In Staff Site
Journal:
Microscopy and Microanalysis Cambridge University Press:
Volume:
Keywords :
Tramadol Promotes Oxidative Stress, Fibrosis, Apoptosis,
Abstract:
Tramadol is a centrally acting analgesic drug, used for the management of moderate to severe pain in a variety of diseases. The long-term use of tramadol can induce endocrinopathy. This study aimed to evaluate the effect of tramadol dependence on the adrenal cortex and the effect of its withdrawal. Thirty adult male rats were divided into three experimental groups: the control group, the tramadol-dependent group that received increasing therapeutic doses of tramadol orally for 1 month, and the recovery group that received tramadol in a dose and duration similar to the previous group followed by a withdrawal period for another month. Specimens from the adrenal cortex were processed for histological, immunohistochemical, enzyme assay, and quantitative real-time PCR (RT-qPCR) studies. Tramadol induced a significant increase in malondialdehyde level and a significant decrease in the levels of glutathione peroxidase and superoxide dismutase. A significant decrease in the levels of adrenocorticotrophic hormones, aldosterone, cortisol, corticosterone, and dehydroepiandrosterone sulfate was also detected. Severe histopathological changes in the adrenal cortex were demonstrated in the form of disturbed architecture, swollen cells, and shrunken cells with pyknotic nuclei. Inflammatory cellular infiltration and variable-sized homogenized areas were also detected. A significant increase in P53 and Bax immunoreaction was detected and confirmed by RT-qPCR. The ultrastructural examination showed irregular, shrunken adrenocorticocytes with dense nuclei. Dilated smooth endoplasmic reticulum, mitochondria with disrupted cristae, and numerous coalesced lipid droplets were also demonstrated. All these changes started to return to normal after the withdrawal of tramadol. Thus, it was confirmed that the long-term use of tramadol can induce severe adrenal changes with subsequent insufficiency
Author Related Publications
Mohamed Ali Ahmed Mohamed Alabiad, "The Effect of Induced Diabetes Mellitus on the Cerebellar Cortex of Adult Male Rat and the Possible Protective Role of Oxymatrine: A Histological, Immunohistochemical and Biochemical Study", Informa Healthcare, 2021
More
Mohamed Ali Ahmed Mohamed Alabiad, "Prognostic and clinicopathological implications of expression of Beclin-1 and hypoxia-inducible factor 1α in serous ovarian carcinoma: an immunohistochemical study", Versalius University Medical Publisher, 2021
More
Mohamed Ali Ahmed Mohamed Alabiad, "Prognostic and clinicopathological significance of TMEFF2, SMOC-2, and SOX17 expression in endometrial carcinoma", Academic Press Inc., 2021
More
Mohamed Ali Ahmed Mohamed Alabiad, "Metanil yellow promotes oxidative stress, astrogliosis, and apoptosis in the cerebellar cortex of adult male rat with possible protective effect of scutellarin: A histological and immunohistochemical study", Elsevier Ltd., 2021
More
Mohamed Ali Ahmed Mohamed Alabiad, "Combined lead and zinc oxide‐nanoparticles induced thyroid toxicity through 8‐OHdG oxidative stress‐mediated inflammation, apoptosis, and Nrf2 activation in rats", John Wiley and Sons Inc., 2021
More
Department Related Publications
Amal Mohamed Mahmoud Mangoud, "- Initiation of Cytological Diagnosis Unit 1 /1 /1984 المجلة المجلة", لايوجد, 1900
More
Amal Mohamed Mahmoud Mangoud, "- Tissue Immunity in Rhinoscleroma 1 /3 /1986 المجلة المجلة", لايوجد, 1900
More
Amal Mohamed Mahmoud Mangoud, "Neprotic Syndrome: An Etiological, Histopathological, Immunological and Ultrastruchral Study 1 /1 /1992 المجلة المجلة", لايوجد, 1900
More
Amal Mohamed Mahmoud Mangoud, "AgNor Regions Distribution in Diagnosis of Premalignant and Malignant Lesions in the Urinary Bladder 1 /8 /1994 المجلة المجلة", لايوجد, 1900
More
Amal Mohamed Mahmoud Mangoud, "Imaging of the Endometrium in Dysfunctional Uterine Bleeding by Electron Microscopy 1 /8 /1994 المجلة المجلة", لايوجد, 1900
More
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف