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Micropropagation, Myristicin Production Enhancement, and Comparative GC-MS Analysis of the n-Hexane Extracts of Different Organs of Daucus pumilus (Gouan), Family Apiaceae
Faculty
Pharmacy
Year:
2020
Type of Publication:
ZU Hosted
Pages:
Authors:
Sahar Abdelaziz Mohamed Abdalaziz
Staff Zu Site
Abstract In Staff Site
Journal:
Journal of Pharmacy and Bioallied Sciences medknow
Volume:
Keywords :
Micropropagation, Myristicin Production Enhancement, , Comparative GC-MS Analysis
Abstract:
Aim: This work aimed to study the somatic embryogenesis and organogenesis of endangered Daucus pumilus (Gouan) for the conservation of this plant and improving the production of secondary metabolites of medicinal value. Materials and Methods: The callus formation and in vitro propagation of D. pumilus (Gouan) by using a different combination of naphthalene acetic acid and benzylaminopurine were established. Various embryogenic stages were tracked using scanning electron microscopy and light microscopy. The volatile constituents of the n-hexane extracts of D. pumilus (Gouan) that extracted by ultrasonic-assisted technique were analyzed by gas chromatography–mass spectrometry. Results and Discussion: Somatic embryogenesis and organogenesis of endangered D. pumilus (Gouan) were established for the first time. Myristicin and elemicin were successfully increased during micropropagation to 70.89% and 2.19%, respectively. Furthermore, the induction of compounds such as 6-methoxymellein, eugenin, methyl behenate, and 1,6-dimethylnaphthalene was also detected. Conclusion: Commercially, this protocol decreases the dependence on wild medicinal plants, enhances the manufacturing of valuable phytochemicals to meet the great demands of the pharmaceutical industries, and acts as a mean for genetic transformation of this plant.
Author Related Publications
Sahar Abdelaziz Mohamed Abdalaziz, "MACRO- AND MICROMORPHOLOGICAL STUDY OF DAUCUS CAPILLIFOLIUS GILLI CULTIVATED IN EGYPT MACRO- AND MICROMORPHOLOGICAL STUDY OF DAUCUS CAPILLIFOLIUS GILLI CULTIVATED IN EGYPT", European Journal of Pharmaceutical and Medical Research, 2018
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Sahar Abdelaziz Mohamed Abdalaziz, "Anticancer activity and concurrent analysis of ursolic acid, b-sitosterol and lupeol in three different Hibiscus species (aerial parts) by validated HPTLC method", Elsevier, 2018
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Sahar Abdelaziz Mohamed Abdalaziz, "Chemical Composition and Biological Activities of the Aqueous Fraction of Parkinsonea aculeata L. Growing in Saudi Arabia", Elsevier, 2018
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Sahar Abdelaziz Mohamed Abdalaziz, "Expression of Biphenyl Synthase Genes and Formation of Phytoalexin Compounds in Three Fire Blight-Infected Pyrus communis Cultivars", PLOS ONE, 2016
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Sahar Abdelaziz Mohamed Abdalaziz, "Concurrent analysis of bioactive triterpenes oleanolic acid and b-amyrin in antioxidant active fractions of Hibiscus calyphyllus, Hibiscus deflersii and Hibiscus micranthus grown in Saudi Arabia by applying validated HPTLC method", Elsevier, 2017
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Department Related Publications
Ehsan Mahmoud Abdelaziz Abozied, "12) Chemical Constituents of Gomphocarpus Sinaicus Boiss, Family Asclepiadceae.Growing in Egypt", لايوجد, 1900
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Ehsan Mahmoud Abdelaziz Abozied, "13) Macro- and Micromorphology of the Flower and Fruit of Gomphocarpus Sinaicus Boiss. Family Asclepiadaceae. Growing in Egypt", لايوجد, 1900
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Ehsan Mahmoud Abdelaziz Abozied, "29) Improved Heigh-preformance liquid chromatographic Determination of Glycyrrhizin in Licorice and pharmaceutical preparations", لايوجد, 1900
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Samih Ibrahim Ahmed Eldhmy , "pharmacognostical Study of Some Plants Belonging to Compositae", لايوجد, 1900
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Afaf Alsayed Ali Abdelghanie, "A New Quantitative Method for Estimation of Haemolytic Saponins in Plants", لايوجد, 1900
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