Role of glutathione metabolism in relation to nephrotoxicity

Faculty Veterinary Medicine Year: 2008
Type of Publication: Theses Pages: 137
Authors:
BibID 10179516
Keywords : Veterinary Biochemistry    
Abstract:
A total of 60 Adult male Sprague-Daley rat weighting 200-250gm were used in this Experiment. This animals were obtained from the Centeral Animals House of Faculty of Veterinary MedicineZigzag University. Animals were maintained in stainless steel cages inthe biochemistry Department under. hygienic condition. The animalsreceived standard rats pellet diet and water labiturh. Rats under studywere randomly divided in to six groups according to treatment. The rats were acclimatized for 2 days and randomly divided to six groups of ten animals each set up as follow: The animals were divided into SIX groups of ten rats each.Group I was injected with saline intraperitoneally. This group servedas control. Groups 11 was treated with GSH for 15 days and then received CP plus GSH for 5 days., Groups III was treated with CV for 15 days then injected with CP plus VC for 5 days. Groups IV was treated with CP plus GSH for 5 days. This group treated wiGroup VI was treated with CP only for 5 days. Blood samples werecollected in heparinized tubes from the rats killed 7 days after Cl’ orsaline administration and their kidneys were removed and kept in anice bath until homogenization. Serum was used for the estimation ofurea and creatinine and kidney tissue for the determination of contents1. The final live body weight of New Zealand white rabbit at 8 weeks of age for the different treatment groups were 2230, 2347, 2537.1, 2248.3, 2300, 2236.6 and 2277.5 gm. The result showed that the highest body weight of group that fed on 15% olive cake 2. Result showed no significant difference in body gain but group that fed on 15% olive cake meal + enzyme provided the best body gain (233.01) gm.3. Results showed that feed consumption not affected significantly.4. Results showed that feed conversion ratio affected significantly and group that fed on 15% olive cake meal + enzyme provided the best value (3.09). Also, all treated groups showed good value when compared with control.B. Blood parameter:5. Results showed that total protein not affected significantly but albumin and globulin and A/G ratio affected significantly.6. Results showed a significant differences in cholesterol and triglyceride level and group that fed on 15% sugar beet pulp + enzyme and group that fed on 15% olive cake meal + enzyme recorded the lowest value (68.66 , 86.66 mg/100 ml) for cholesterol andC. Digestibility trials:7. Feeding rabbits on diet containing OCM, SBP and dried brewers grain in their ration caused increase in digestion coefficient of DM, OM and CP the highest value were (68.74, 69.44 and 73.02%) of group that fed on15% OCM+ enzyme respectively. Also, all t8. The higher digestion coefficient of CF and EE were (47.63 and 77.35%) respectively of group that fed on15% dried brewer’s grain + enzyme9. The higher digestion coefficient of NFE was (76.03%) of group that fed 15% dried brewer’s grainD. Caecum activity:10. The results showed that the highest caecum pH value was (6) of group fed 15% OCM + enzyme but the lowest value was (5.26) of control group.11.Results showed a significant differences in ammonia and total volatile fatty acids the highest value of ammonia was (12.60mg/100ml) of group that fed on15% OCM + enzyme but the lowest value was (11.20 mg/100 ml) of group that fed on 15% dried brewer grE. Carcass traits:12. The result showed that the highest live body weight and carcass weight were (2588.66 and 1423.66 gm) of group that fed on15% OCM + enzyme respectively but the lowest value (2218.33 and 1097.33 gm) of control group. Also, all treated group provided car13. Group that fed on 15% OCM + enzyme provided the highest dressing percentage (54.98) and the results showed a significant difference.14. There is a significant difference in liver, kidney, lung and heart weights. The highest liver weight was of group that fed on15% OCM + enzyme (64.0gm) and lowest value was (34.0gm) of control the highest kidney, lung and heart weights were (19.33, 17.15. The highest edible part was (59.11%) of group that fed on15% OCM + enzyme and the lowest was of control (53.29%).F. Economical efficiency:16. The result showed that rations containing 15% OCM with or without enzyme, 15% SBP with or without enzyme and 15% DBG with or without enzyme provided the lowest total costs required to produce one kilogram of live body weight or body weight gain.Summary:-The experimental work of the present study was carried out in the rabbit’s farm of Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt from October to December 2008.A total number of 56 weanling New Zealand rabbits of 35 day old, were divided into seven groups (8 each). The first group was used as a control and other groups supplemented with different types of feed with addition of enzyme to some groups as following The experimental rations were offered ad \libitum twice daily. Rabbits were individually weighted to the nearest gram every week. At the end of experimental period, three representative rabbit’s samples from each feeding group were randomly taken to studyTen digestion trials were conducted to determine the digestion coefficient of nutrients and nutritive value of the experimental diets.Also, three caecum samples from each group after slaughter were taken to determine cecum pH, Ammonia and total volatile fatty acid.The obtained results are summarized as follows:A. Growth performance:1. The final live body weight of New Zealand white rabbit at 8 weeks of age for the different treatment groups were 2230, 2347, 2537.1, 2248.3, 2300, 2236.6 and 2277.5 gm. The result showed that the highest body weight of group that fed on 15% olive cake 2. Result showed no significant difference in body gain but group that fed on 15% olive cake meal + enzyme provided the best body gain (233.01) gm.3. Results showed that feed consumption not affected significantly.4. Results showed that feed conversion ratio affected significantly and group that fed on 15% olive cake meal + enzyme provided the best value (3.09). Also, all treated groups showed good value when compared with control.B. Blood parameter:5. Results showed that total protein not affected significantly but albumin and globulin and A/G ratio affected significantly.6. Results showed a significant differences in cholesterol and triglyceride level and group that fed on 15% sugar beet pulp + enzyme and group that fed on 15% olive cake meal + enzyme recorded the lowest value (68.66 , 86.66 mg/100 ml) for cholesterol andC. Digestibility trials:7. Feeding rabbits on diet containing OCM, SBP and dried brewers grain in their ration caused increase in digestion coefficient of DM, OM and CP the highest value were (68.74, 69.44 and 73.02%) of group that fed on15% OCM+ enzyme respectively. Also, all t8. The higher digestion coefficient of CF and EE were (47.63 and 77.35%) respectively of group that fed on15% dried brewer’s grain + enzyme9. The higher digestion coefficient of NFE was (76.03%) of group that fed 15% dried brewer’s grainD. Caecum activity:10. The results showed that the highest caecum pH value was (6) of group fed 15% OCM + enzyme but the lowest value was (5.26) of control group.11.Results showed a significant differences in ammonia and total volatile fatty acids the highest value of ammonia was (12.60mg/100ml) of group that fed on15% OCM + enzyme but the lowest value was (11.20 mg/100 ml) of group that fed on 15% dried brewer grE. Carcass traits:12. The result showed that the highest live body weight and carcass weight were (2588.66 and 1423.66 gm) of group that fed on15% OCM + enzyme respectively but the lowest value (2218.33 and 1097.33 gm) of control group. Also, all treated group provided car13. Group that fed on 15% OCM + enzyme provided the highest dressing percentage (54.98) and the results showed a significant difference.14. There is a significant difference in liver, kidney, lung and heart weights. The highest liver weight was of group that fed on15% OCM + enzyme (64.0gm) and lowest value was (34.0gm) of control the highest kidney, lung and heart weights were (19.33, 17.15. The highest edible part was (59.11%) of group that fed on15% OCM + enzyme and the lowest was of control (53.29%).F. Economical efficiency:16. The result showed that rations containing 15% OCM with or without enzyme, 15% SBP with or without enzyme and 15% DBG with or without enzyme provided the lowest total costs required to produce one kilogram of live body weight or body weight gain. 
   
     
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