This study was designed to record the prevalence rate of hydatidosis among farm animals and human in Najran area. Experiment I recorded the rate of hydatidosis in camels, sheep and goats. A total of 48139 animals (camels = 4531, sheep= 29916, goats= 13692) were employed in this experiment. The animals were examined at post- mortem for the presence of hydatid cysts. The results showed that the rate of prevalence differs (p<0.001) among animal species with higher rates of infection in sheep (6.8%), camels (5.4%) and goats (2.2%). The overall rate of prevalence was 5.3%. Experiment II is carried to determine the prevalence of hydatidosis in human. At total of 1142 human serum samples were tested with ELISA and IHA (ELISA =276, IHA=866) for the presence of hydatid antibodies. Fifty two serum samples (ELIZA = 17, IHA= 35) were positive for hydatid antibodies (4.5%) with no significant difference (p >0.05) between ELISA and IHA results. However, out of 57 negative samples evaluated with IHA, 4 samples were positive when tested with ELISA and out of 35 IHA positive samples 4 samples were negative when tested with ELISA. Consequently, the sensitivity, specificity and accuracy of IHA were 88.5 %, 92.9% and 91.3%, respectively. In conclusion hydatidosis prevails in Najran area at an overall rate of 5.3% in farm animals and 4.5% in human. Additionally, ELIZA and/or IHA can be used to screen hydaitdosis in human.