Leishmaniasis is of a veterinary and zoonotic importance. This study asserted the sheep and goat carriers of Visceral Leishmaniasis in Ismailia and Sharqia Governorate, Egypt. Screening of domestic animals, sheep and goats was carried out to detect antibodies against Leishmania donovani and to see the involvement of animal reservoirs. A total of 513 domestic animals were included in the study. Serum samples were collected and surveyed by indirect hemagglutination assay (IHAT). The overall seroprevalence of sheep and goats Leishmaniasis infection in Ismailia and Sharqia Governorate, Egypt was 7.6 % (39/513).Seroprevalence of sheep and goat Leishmaniasis in Sharqia Governorate was 7.1 % (15/210) while in Ismailia Governorate was 7.9% (24/303). The highest titer in sheep and goat leishmanisis was 1/512 with percentage of 20.5%. The overall prevalence rate of Leishmaniasis infection in Ismailia and Sharqia Governorate was significantly higher (P<0.05) in old age and females than young age and males. The study was thrown light on the possibility of varied species of domestic animals to harbor the parasite and hence play a central role in the Leishmaniasis transmission in Ismailia and Sharqia Governorate, Egypt. Consequently, this may hurdle our clarification of disease dynamics.