This work aimed to study the role of nitric oxide in ischemia andreperfusion injury in renal transplanted rat model.Rats were first undergoes kidney transplantation according to Zheng etGroup 3: Healthy buffalo calves intramuscular injected with half therapeutic dose of boldenone undecylenate (0.55/kg b. wt.)Group 4: Healthy buffalo calves intramuscular injected with therapeutic dose of boldenone undecylenate( 1.1mg /kg b.wt.)Two blood samples were collected 6 times at 4 weeks intervals post application of probiotic and boldenone undecylenate from calves of all groups. 1st Sample was taken in tube contain EDTA and used for