In this study seventy buffaloes were examinedwhich consists of four groups.Group I was twenty appearently healthy buffaloes, agingfrom )-7 year and fed on berseem, concentrammixture,rice straw and mineral mixture.Group II was Twenty buffaloes which showed anestrous,aging from 4-11 year and fed on wheat straw and drawa~or dr awa and c onc en tr ate mixtur e •Group III was fifteen buffaloes showed indigestion,aging from 8 mounth to 10 year and fed on berseem orberseem and whes.t straw or berseem, tiben and wheatbran.Group IVwas fifteen buffaloes showed sYmptoms ofalopecia, aging from 2% 5 year and fed on berseem andrice straw.In all animals both serum and diet were used, fordetermination of oalcium, inorganic phosphorus and copperas well as ca/p ratio.1. In buffaloes affected with anestrum: There werean elevation of serum calcium as well as a highly