The present study was carried out on 45 clinically healthydogs of different age, sex and body weight.All animals were operated u~on for cholecystectomy throughabdominal approach the animals were divided into 3 groups, eachof 15 dogs, ar c or-d.l ng to the site of laparotomy the incision wasde ne namely; midline, parailledian and cresoentic ,araoostal.In addition to the surgical-techmique, the present work wasaimed to study some serum biochemical variations and pathoanatomical and histopahological findings were also recerded beforeand after operations.All operated dogs withstood well the surgical interventionand manifested no post operative conilcations during the CDurseof the experiments that lasted for 30 - 60 days •The abdominal ih digs of all groups healed by primaryintenti~n eXc8pt tow cases in which the healing process was retardedfrom the results of the present experiments, it becameCladosporcums pp , , 1.1ucor Sp?, rrr:’chodernn spp 0 , !’~l teD!!riaspp , , Rhizopus spp Q , Geot:richumcand i.dum , Cenfjp,los”)oriumspp , t Fusaril.un ~~pe9i.;es, rrharrmidium spp 0, Botryt is app , ,-Syncepr.alo.strum spp •., ]?llllularia~,Sporotrichum ci:irnis,MoniliFt s nn , and He Imint ho e oo r-Lum s pp <> could be Lso 10. tea 11 tvarying percentages ranging from 2% to 78% of e r am Lne d samples.Further iclentificat ion of t.so La tee. l:.sper,3illusandPenicillium speedos were recorded as follows ~ nigor ,~. flavus, . A_ 0 fumige.tus ,~ sodywi, ~. olavatusA_ 0 cadidus, A ... versicolor, A .• re pans and _ A • terrus ,while Penicillium species reported v:ere P. verrucosum varcyclopium ’.! ... nigerice.ns , p”, chrysor:enum , P <> oxalicum ,P. grisofulvum , P. br-ev Lcompac t um t P .. rE;~ueforti , Po expan- -- --sum , P; vir:i.dicatum , P •• albidum and p” mu Lt Lco Lo r all were -isola ted from frozen meat”Frozen chicken samples examined for mould contamination