Genetic Analysis For Salt Tolerance of Some Mutant Lines in Faba Been (Vicia Faba L.)

Genetic evaluation was performed on ten faba bean genoypes (Vicia faba L.) for salt tolerance. Salt tolerance related criteria, used in this study, were, seed germination, plant height, osmotic pressure at 30 days and 60 days and proline content at 60 days. The obtained results confirmed more variabilities between mutant lines and varities under study. The LP7 new promising line was the most salt tolerant than other genotypes, while, Giza 714 considered the most salt sensitive in all studied criteria. Osmotic pressure at 30 , 60 days and proline content recorded the highest values in LP7 and subsequently decreased in the other tested genotypes. The results showed also that a positive correlation had been found between seed germination % and plant height at 30 days, osmotic pressure at 30 days and proline content under 5000 ppm.Analysis of variance for stability of morphological and biochemical characters among faba bean genotypes under saline environment interactions for all attributes except seed germination showed genetic differences among these entries for their regression on the environmental indices.Accordingly, LP7 as a tolerant line and G714 as a sensitive cultivar were crossed for studying molecular markers related to salt tolerance by using bulked segregant analysis (BSA). The RAPD-PCR products exihibited seven positive molecular markers in the tolerant parent and eight negative molecular markers in the sensitive parent. These results showed the possibility of genotypes for salt tolerance in breeding faba bean using molecular markers.